ZymoPURE - Express Plasmid Midiprep Kit


ZymoPURE - Express Plasmid Midiprep Kit

Cat # Name Size Price Quantity
D4213 ZymoPURE - Express Plasmid Midiprep Kit 25 Preps. €287,00
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ZymoPURE - Express Plasmid Midiprep Kit


  • Pellet-Free: Direct lysis procedure omits cell-pelleting, resuspension steps, and large centrifuges.
  • Quick & Pure: 15 minutes from culture flask to transfection-grade plasmid DNA.
  • Highest Yield: Purify up to 1.2 mg of plasmid DNA using a spin-column.

The ZymoPURE - Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE - Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE - Express Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing. No large centrifuge! No cell pelleting! No gravity filtration! No ethanol precipitation! From culture to endotoxin-free plasmid DNA in 15 minutes!

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Applicable For Eluted DNA is well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.
Binding Capacity 1.2 mg
Culture Input ≤ 25 mL
Elution Volume ≥ 200 µl
Endotoxin Levels ≤1 EU/µg Plasmid DNA
Equipment Microcentrifuge and vacuum/vacuum manifold
Format Spin Column
Processing Time 15 min
Purity Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0
Recommended Sample Volume ≤ 25 ml
Size Range Up to 200 kb
Yield ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized). 1.2 mg maximum binding capacity of column.

Q1: I accidently left my ZP Express Neutralization Buffer at room temperature, is it still stable for use?

Yes, the RNase A is fairly stable at room temperature but we recommend placing it in 4 °C as soon as possible.

Q2: Can you recover up to 1.2 mg DNA with the ZymoPURE-Express Plasmid Midiprep Kit?

The binding capacity of the column is up 1.2 mg DNA, however, typical yields from directly processing 25 ml culture of a high copy plasmid is 100 – 200 ug total DNA.

Q3: Can you process more than 25 ml culture?

Yes, up to 50 ml bacterial culture in LB Medium can be pelleted, resuspended, and processed with the kit (see appendix in instruction manual).

Q4: What is the composition of the ZymoPURE Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Cat # Name Size Price
D4200-5-55 ZymoPURE Wash 1 55 ml €40,00
D4200-6-23 ZymoPURE Wash 2 (Concentrate) 23 ml €40,00
D4200-7-12 ZymoPURE Elution Buffer 12 ml €20,00
D4213-2-260 ZP Express Neutralization Buffer 260 ml €75,00
D4213-1-210 ZP Express Lysis Buffer 210 ml €243,00
D4213-3-260 ZP Express Binding Buffer 260 ml €72,00
C1036-5 ZymoPURE Syringe Filters 5 Pack €28,00
C1037-5 ZymoPURE Syringe Plungers 5 Pack €14,00
C1042-5 Zymo-Spin V-P Column Assembly w/ 15 ml and 50 ml Reservoir 5 Pack €66,00