Fully customizable services for chromatin immunoprecipitation followed by next-generation sequencing
- High-quality data generation with optimized workflows and customized analysis
- Full service analysis includes the ChIP assay, library prep, NextGen sequencing, and bioinformatics
- Compatible with low sample inputs
- Rapid turnaround times at an economical price
ChIP-Seq is a powerful tool for genomewide mapping of histone modifications, proteinDNA interactions, and identifying consensus proteinbinding sites in DNA. With the ChIPSeq service from Zymo Research, you can either perform the ChIP assay yourself and send us the enriched DNA for library construction and NextGen sequencing, or we can process your samples using our optimized, proprietary chromatin shearing and enrichment procedures. We also perform the bioinformatics and statistical analyses, and send you the results in publicationready format.
This platform (an improved version of Reduced Representation Bisulfite Sequencing for greater coverage) can be used to detect 3 4 million unique CpG sites, allowing >85% coverage of all CpG islands and >80% of all gene promoters for a maximal amount of methylation data from less sequencing reads, reducing the overall cost. The system is conducive to biomarker discovery by providing for the identification and analysis of differentially methylated regions (DMRs) between samples.
MidiSeq can be used to detect 8-9 million unique CpG sites. It extends the coverage of the Methyl-MiniSeq platform to include genetic regulatory elements, gene bodies, and repeated DNA sequences. It is a good option for those researchers requiring methylome analysis outside of gene promoters and CpG islands.
The Methyl-MaxiSeq platform (whole-genome bisulfite sequencing) is for the detection of DNA methylation across the entire genome. DNA methylation information is provided in CpG context as well as in the less common CHG and CHH contexts. The platform attains an average read coverage of 15-20X per base (for the human genome). This can be modified depending on your requirements. Since whole-genome sequence is provided, SNP analysis can be performed simultaneously.