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Propagation Strains
Expression Strains

Propagation Strains

Ideal for cloning, plasmid isolation, and library construction

JM109 DH5 Alpha HB101 TG1 Zymo 10B
Specifications
Strain Background K-12 K-12 K-12 K-12 K-12
General Cloning
Plasmid Isolation
Recombinant Protein Expression
Production of ssDNA (F' episome)
Suppression of Amber Mutations (glnV44 or supE44)
Blue-White Selection (lacZΔM15)
High-quality and Yield of Plasmid DNA (endA1)
Reduced Recombination & Insert Stability (recA1 or recA13)
Plasmid Size Up to 10-15 kb Up to 10-15 kb Up to 10-15 kb
Transformation of Large Plasmids (deoR) Up to 20-32 kb Up to 20-32 kb
Ampicillin Resistant (bla or ampR)
Chloramphenicol Resistant (cat or CmR or CamR)
Tetracycline Resistant (Tn10 or tetR)
Kanamycin Resistant (KanR)
Nalidixic Acid Resistant (gyrA96 or NalR)
Streptomycin Resistant (StrR)
Genotype F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 F- φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1, λ- F- Δ(gpt-proA)62 leuB6 glnV44 (supE44) ara-14 galK2 lacY1 Δ(mcrC-mrr) xyl-5 mtl-1 recA13 thi-1 rpsL20 (SmR) F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB) F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ-
Catalog Number T3003 T3007 T3011 T3017 T3019

Expression Strains

Ideal for protein expression and DNA analysis

XJa
Autolysis
XJa (DE3)
Autolysis
XJb Autolysis XJb (DE3)
Autolysis
Specifications
Strain Background K-12 K-12 B B
General Cloning
Plasmid Isolation
General Screening
Recombinant Protein Expression
Production of ssDNA (F' episome)
T7 Promoter Transcription (λDE3)
Autolysis (ΔaraB::λR) Autolysis Inducible
by Arabinose
Autolysis Inducible
by Arabinose
Autolysis Inducible
by Arabinose
Autolysis Inducible
by Arabinose
Suppression of Amber Mutations (glnV44 or supE44)
Blue-White Selection (lacZΔM15)
High-quality and Yield of Plasmid DNA (endA1)
Reduced Recombination & Insert Stability (recA1 or recA13)
Plasmid Size Up to 10 kb Up to 10 kb Up to 10 kb Up to 10 kb
Transformation of Large Plasmids (deoR)
Ampicillin Resistant (bla or ampR)
Chloramphenicol Resistant (cat or CmR or CamR)
Tetracycline Resistant (Tn10 or tetR)
Kanamycin Resistant (KanR)
Nalidixic Acid Resistant (gyrA96 or NalR)
Streptomycin Resistant (StrR)
Genotype F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR) F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44)e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR), λ(DE3) F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR) F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR), λ(DE3)
Catalog Number T3021/T5021 T3031/T5031 T3041/T5041 T3051/T5051

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