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Propagation Strains
Expression Strains
Propagation Strains
Ideal for cloning, plasmid isolation, and library construction
JM109 | DH5 Alpha | HB101 | TG1 | Zymo 10B | |
---|---|---|---|---|---|
Specifications | |||||
Strain Background | K-12 | K-12 | K-12 | K-12 | K-12 |
General Cloning | ✓ | ✓ | ✓ | ✓ | ✓ |
Plasmid Isolation | ✓ | ✓ | ✓ | ✓ | ✓ |
Recombinant Protein Expression | ✓ | ||||
Production of ssDNA (F' episome) | ✓ | ✓ | |||
Suppression of Amber Mutations (glnV44 or supE44) | ✓ | ✓ | ✓ | ✓ | |
Blue-White Selection (lacZΔM15) | ✓ | ✓ | ✓ | ✓ | |
High-quality and Yield of Plasmid DNA (endA1) | ✓ | ✓ | ✓ | ||
Reduced Recombination & Insert Stability (recA1 or recA13) | ✓ | ✓ | ✓ | ✓ | |
Plasmid Size | Up to 10-15 kb | Up to 10-15 kb | Up to 10-15 kb | ||
Transformation of Large Plasmids (deoR) | Up to 20-32 kb | Up to 20-32 kb | |||
Ampicillin Resistant (bla or ampR) | |||||
Chloramphenicol Resistant (cat or CmR or CamR) | |||||
Tetracycline Resistant (Tn10 or tetR) | |||||
Kanamycin Resistant (KanR) | |||||
Nalidixic Acid Resistant (gyrA96 or NalR) | ✓ | ✓ | |||
Streptomycin Resistant (StrR) | ✓ | ✓ | |||
Genotype | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rk- mk+) relA1 recA1 | F- φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1, λ- | F- Δ(gpt-proA)62 leuB6 glnV44 (supE44) ara-14 galK2 lacY1 Δ(mcrC-mrr) xyl-5 mtl-1 recA13 thi-1 rpsL20 (SmR) | F’[traD36 lacIq Δ(lacZ) M15 proA+B+] glnV (supE) thi-1 Δ(mcrB-hsdSM)5 (rK- mK- McrB-) thi Δ(lac-proAB) | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- |
Catalog Number | T3003 | T3007 | T3011 | T3017 | T3019 |
Expression Strains
Ideal for protein expression and DNA analysis
XJa Autolysis™ |
XJa (DE3) Autolysis™ |
XJb Autolysis™ | XJb (DE3) Autolysis™ |
|
---|---|---|---|---|
Specifications | ||||
Strain Background | K-12 | K-12 | B | B |
General Cloning | ✓ | ✓ | ||
Plasmid Isolation | ✓ | ✓ | ||
General Screening | ✓ | ✓ | ||
Recombinant Protein Expression | ✓ | ✓ | ✓ | ✓ |
Production of ssDNA (F' episome) | ✓ | ✓ | ||
T7 Promoter Transcription (λDE3) | ✓ | ✓ | ||
Autolysis (ΔaraB::λR) | Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
Autolysis Inducible by Arabinose |
Suppression of Amber Mutations (glnV44 or supE44) | ✓ | ✓ | ||
Blue-White Selection (lacZΔM15) | ✓ | ✓ | ||
High-quality and Yield of Plasmid DNA (endA1) | ✓ | ✓ | ||
Reduced Recombination & Insert Stability (recA1 or recA13) | ✓ | ✓ | ||
Plasmid Size | Up to 10 kb | Up to 10 kb | Up to 10 kb | Up to 10 kb |
Transformation of Large Plasmids (deoR) | ||||
Ampicillin Resistant (bla or ampR) | ||||
Chloramphenicol Resistant (cat or CmR or CamR) | ✓ | ✓ | ✓ | ✓ |
Tetracycline Resistant (Tn10 or tetR) | ||||
Kanamycin Resistant (KanR) | ||||
Nalidixic Acid Resistant (gyrA96 or NalR) | ||||
Streptomycin Resistant (StrR) | ||||
Genotype | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44) e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR) | F`[traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 (supE44)e14- (McrA-) thi gyrA96 (NalR) endA1 hsdR17(rK- mK+) relA1 recA1 ΔaraB::λR, cat (CmR), λ(DE3) | F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR) | F- ompT hsdSB(rB- mB-) gal dcm ΔaraB::λR, cat (CmR), λ(DE3) |
Catalog Number | T3021/T5021 | T3031/T5031 | T3041/T5041 | T3051/T5051 |
Custom & OEM Solutions
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