- Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
- High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
- Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
|Additional Info||Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.|
|Genotype||F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ-|
|Processing Time||20 Seconds|
|Product Storage||-70°C to -80°C|
|Transformation Efficiency||108 - 109 transformants per µg of plasmid DNA|
Q1: Are competent cells GMOs?
Q2: Are the Mix & Go! strains dam+ and dcm+?
Q3: Do the Mix & Go! strains methylate DNA?
Q4: Which strains are equivalent to the Zymo strains?
Q5: How to reduce satellite colonies on agar plates?
Q6: Is it possible to dilute the competent cells?
Q7: Which antibiotics can be used with the Mix & Go! procedure?
Q8: Which Plasmid Size can be used for transformation?
Q9: Which is the recommended DNA concentration and volume for transformation?
Q10: What are some tips to improve transformation efficiency?
Q11: How will a heat-shock affect my Transformation Efficiency?
The mechanisms of electrotransfection of mammalian cells were determined by observing the interaction of plasmid DNA (pDNA) with the cell membrane and internalization of the pDNA into the cell. The plasmid used in the study was amplified using Mix & Go E. coli Competent Cells, strain Zymo 5α, from Zymo Research, and labeled with a fluorescence dye to visualize pDNA binding and internalization. The authors found that efficient electrotransfection is dependent on the presence of divalent cations and endocytic pathways that can transport the pDNA across the membrane.Wu, M. and F. Yuan. (2011) Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cell. PloS One 6:e20923.
In the process of developing a protocol to facilitate real-time imaging of apoptotic cell membrane changes in live cells, researchers used the Mix & Go Z-competent E.coli strain Zymo 5α from Zymo Research for fast, high-efficiency transformations to express annexin B12 modified with fluorophores that fluoresce when bound to phosphatidylserine-containing membranes.Kim, Y.E. et al. (2010) Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis. Nature Protocols 5: 1396-1405.