|Applicable For||Next-Generation Sequencing, qPCR, microarray.|
|Reagent Storage||Ambient temperature => 2 years|
|Sample Collection||10% (v/v or w/v)|
|Sample Source||Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.|
|Sample Stability||RNA: Ambient temperature (4°C- 25°C) >1 month
DNA: Ambient temperature (4°C- 25°C) >2 years
DNA & RNA: Frozen (<-20°C): Indefinitely
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
Q3: How to collect solid tissues in DNA/RNA Shield?
Q4: Can I FACS sort directly into DNA/RNA Shield?
Q5: How long can samples be stored frozen in DNA/RNA Shield?
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
Q7: What sample types is DNA/RNA Shield suitable for?
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
A robust and sensitive assay was developed to screen for the 18S rRNA gene of Plasmodium falciparum and Plasmodium vivax from asymptomatic and low density infections. The new RT-PCR based method could detect malaria infections that were significantly lower than the standard microscopy and rapid detection method. DNA/RNA Shield was used to preserve patient’s blood samples in the field for 14 days at 28°C and 80% humidity.Adams M, et. al. (2015). An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low‑density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples. Malar. J. 14:520.
An RNA-based detection method was developed to detect low asymptomatic malaria infections in rural areas of Haiti. Three different detection methods were utilized--rapid diagnostic test, thick smear microscopy and a qRT-PCR assay were evaluated. The blood samples used for qRT-PCR were preserved in DNA/RNA Shield to maintain the sample integrity until RNA isolation. The qRT-PCR method was the most sensitive and identified significantly more samples.Elbadry MA, et. al. (2015). High prevalence of asymptomatic malaria infections: a cross‑sectional study in rural areas in six departments in Haiti. Malar. J. 14:510.
During a survey of Middle East respiratory syndrome coronavirus (MERS-CoV), nasal swabs from dromedary camels were collected into DNA/RNA Shield . Any MERS-positive swab samples were completely inactivated in DNA/RNA Shield and were subsequently used for RNA extraction. Successful detection of MERS-CoV and phylogenetic analysis suggests local zoonotic transmission through the respiratory route onto humans.Nowotny N, et. al. (2014). Middle east Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels, Oman, 2013. Eurosurveillance. 19(16).
“I did an assay using RNA/DNA shield and it works perfect. The product has excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4 or -20 for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)