DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the genetic integrity and expression profiles of samples at ambient temperatures and completely inactivates infectious agents (viruses, bacteria, fungi, & parasites). The unique DNA and RNA stabilization solution also prevents degradation from freeze-thaw cycling and unexpected freezer failures.
|Applicable For||Next-Generation Sequencing, qPCR, microarray.|
|Reagent Storage||Ambient temperature => 2 years|
|Sample Collection||10% (v/v or w/v)|
|Sample Source||Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.|
|Sample Stability||RNA: Ambient temperature (4°C- 25°C) >1 month
DNA: Ambient temperature (4°C- 25°C) >2 years
DNA & RNA: Frozen (<-20°C): Indefinitely
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q3: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q4: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ul of FACS sorted cells into minimally 400 ul of DNA/RNA Shield).
Q5: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month DNA > 2 years
Q7: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
“I did an assay using RNA/DNA shield and it works perfect. The product has excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4 or -20 for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)