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| Cat # | Name | Size | Price | Quantity |
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Description
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Technical Specifications
| Applicable For | Next-Generation Sequencing, qPCR, microarray. |
|---|---|
| Device Specs | Reagent |
| Reagent Storage | Ambient temperature => 2 years |
| Sample Collection | 10% (v/v or w/v) |
| Sample Source | Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc. |
| Sample Stability | RNA: Ambient temperature (4°C- 25°C) >1 month DNA: Ambient temperature (4°C- 25°C) >2 years DNA & RNA: Frozen (<-20°C): Indefinitely |
Product FAQ
Q1: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q2: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q3: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q4: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ul of FACS sorted cells into minimally 400 ul of DNA/RNA Shield).
Q5: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month DNA > 2 years
Q7: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Citations
A robust and sensitive assay was developed to screen for the 18S rRNA gene of Plasmodium falciparum and Plasmodium vivax from asymptomatic and low density infections. The new RT-PCR based method could detect malaria infections that were significantly lower than the standard microscopy and rapid detection method. DNA/RNA Shield was used to preserve patient’s blood samples in the field for 14 days at 28°C and 80% humidity.
Adams M, et. al. (2015). An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low‑density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples. Malar. J. 14:520.An RNA-based detection method was developed to detect low asymptomatic malaria infections in rural areas of Haiti. Three different detection methods were utilized--rapid diagnostic test, thick smear microscopy and a qRT-PCR assay were evaluated. The blood samples used for qRT-PCR were preserved in DNA/RNA Shield to maintain the sample integrity until RNA isolation. The qRT-PCR method was the most sensitive and identified significantly more samples.
Elbadry MA, et. al. (2015). High prevalence of asymptomatic malaria infections: a cross‑sectional study in rural areas in six departments in Haiti. Malar. J. 14:510.During a survey of Middle East respiratory syndrome coronavirus (MERS-CoV), nasal swabs from dromedary camels were collected into DNA/RNA Shield . Any MERS-positive swab samples were completely inactivated in DNA/RNA Shield and were subsequently used for RNA extraction. Successful detection of MERS-CoV and phylogenetic analysis suggests local zoonotic transmission through the respiratory route onto humans.
Nowotny N, et. al. (2014). Middle east Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels, Oman, 2013. Eurosurveillance. 19(16).Read More
West Nile virus positive plasma samples were inactivated in DNA/RNA Shield and resulting viral RNA was detected and identified by qRT-PCR. Phylogenetic comparisons between human and mosquito-derived strains were closely related but not identical which indicates co-circulation in a confined area.
Kolodziejek J, et. al. (2015). West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014. PLoS One. 10(5).DNA/RNA Shield was used to preserve RNA in plasma samples positive for Dengue and Chikungunya virus. Extracted total RNA was used for downstream sequencing for genotyping and phylogenetic analysis. Results revealed two serotypes (DENV-2 and DENV-3) along with CHIKV infection representing the first documented co-infection.
Phommanivong V, et. al. (2016). Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR. Tropical Medicine and Health. 44:24.Samples taken from groundwater supplies in the city of Kabwee, Zambia were preserved and inactivated in DNA/RNA Shield . The samples were then examined for enteric pathogens. 16S rRNA analyses of the samples identified the presence of enteric bacteria contamination with prevalent contamination of Citrobacter freundii and Vibrio cholera.
Sorensen, JPR. (2015). Tracing enteric pathogen contamination in sub-Saharan African groundwater. Science of the Total Environment. 538: 888–895DNA/RNA Shield was used to preserve biofilm samples of Xyella fastidiosa, a gram negative plant-pathogenic bacterium, until RNA isolation for RNA-Seq analyses. The authors reported that during the presence of calcium (Ca) supplementation, transcriptomic changes were observed in cells to promote biofilm growth, while non-supplementation of Ca resulted in the scattering of Xylella fastidiosa.
Parker JK, et. al. (2016). Calcium transcriptionally regulates the biofilm machinery of Xylella fastidiosa to promote continued biofilm development in batch cultures. Environmental Microbiology. 18(5): 1620–1634.DNA/RNA Shield was used to store water collected onto filters and total RNA was isolated. A detailed metatranscriptomic analysis of the microbial communities points towards differing metabolic cycles occurring in anoxic and saline habitats in upper and deep sea regions respectively.
Pachiadaki MG, et. al. (2014). UnveiliMicroRNA expression profiles of 11 African sorghum plants were analyzed under watered and drought conditions to determine the mechanisms involved in drought tolerance. The leaves from sorghum plants exposed to different growing conditions were preserved in DNA/RNA Shield until RNA extraction.
Hamza NB, et. al. (2016). MicroRNA expression profiles in response to drought stress in Sorghum bicolor. Gene Expression Patterns. 20: 88-98.The researchers sought to better understand the physiological importance of the non-sense-mediated mRNA decay (NMD) in murine Sertoli cells. Mice testes were preserved in DNA/RNA Shield and stored RNA isolation. The data revealed that the knockout of Upf2, which is part of the NMD pathway, resulted in testicular atrophy and sterility.
Bao J, et. al. (2015). UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring fidelity of the transcriptome. Development. 142: 352-362.FACS sorted cells derived from disassociated 3D tissue matrix were collected in DNA/RNA Shield for downstream RNA-seq. Transcriptome analyses revealed that modifying crosslinking of hydrogels can optimize expression patterns to correlate with specific brain regions in an engineering 3D neural tissue.
Tekin, H et al. Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature Biomedical Engineering. 2018.DNA/RNA Shield was used to collect biomass samples from bench-scale microbial fuel cells. After bead-beating homogenization, the supernatant was used for automated DNA and RNA extraction on a Promega Maxwell Magnetic Particle Processor with the Maxwell 16 LEV Blood DNA and 16 LEV simplyRNA Blood kits.
Chen, S et al. Methane-driven microbial fuel cells recover energy and mitigate dissolved methane emissions from anaerobic effluents. Environmental Science: Water Research & Technology. 2018.Reviews
“I did an assay using RNA/DNA shield and it works perfect. The product has excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4 or -20 for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
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“We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.”