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Read The Zymo Research PromiseDNA/RNA Shield Saliva Sputum Collection Kit - DX
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Documents
Product Description
Technical Specifications
Applicable For | Next-Generation Sequencing, qPCR, microarray |
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Device Specs | Saliva collection tube; DNA/RNA Shield (2 mL) |
Reagent Storage | Ambient temperature = > 2 years |
Registration Status | FDA- Emergency Use Authorization CE-IVD Marked |
Sample Collection | 2 ml saliva collection |
Sample Source | Saliva/Sputum |
Sample Stability | RNA: Ambient temperature (4°C- 25°C) > 1 month DNA: Ambient temperature (4°C- 25°C) > 2 years DNA & RNA: Frozen (< -20°C): Indefinitely |
Resources
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used to collect saliva and sputum specimens (FDA-cleared for sputum collection only)
Q2: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q3: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q4: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
“We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.”
- Mariska B. (University of Amsterdam)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
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