Mix & Go! Competent Cells-HB101

T3011 / T3013


Mix & Go! Competent Cells-HB101

T3011 / T3013

Cat # Name Size Price Quantity
T3011 Mix & Go! Competent Cells-HB101 10 x 100 µl €135,00
+ -
T3013 Mix & Go! Competent Cells-HB101 96 x 50 µl €494,00
+ -

Documents


For simple, high efficiency transformations without heat shock and lengthy incubations.

Highlights


  • Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
  • High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
  • Versatile: Excellent for general cloning and plasmid isolation.

Description


The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.

Technical Specifications


Additional Info Streptomycin Resistant (StrR) and can not be used for Blue-White Selection.
Genotype  F- Δ(gpt-proA)62 leuB6 glnV44 (supE44) ara-14 galK2 lacY1 Δ(mcrC-mrr) xyl-5 mtl-1 recA13 thi-1 rpsL20 (SmR)
Processing Time 20 Seconds
Product Storage -70°C to -80°C
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA

Product FAQ


Q1: Are the competent cells GMOs?

Q2: Are the Mix & Go! strains dam+ and dcm+?

Q3: Do the Mix & Go! strains methylate DNA?

Q4: Which strains are equivalent to the Zymo strains?

Q5: How to reduce satellite colonies on agar plates?

Q6: Is it possible to dilute the competent cells?

Q7: Which antibiotics can be used with the Mix & Go! procedure?

Q8: Which Plasmid Size can be used for transformation?

Q9: Which is the recommended DNA concentration and volume for transformation?

Q10: What are some tips to improve transformation efficiency?

Q11: How will a heat-shock affect my Transformation Efficiency?

Product Video