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ZymoPURE II Plasmid Purification
Plasmid Purification Reinvented.
See how simple plasmid purification could be.
Don't wait for gravity.
Midi & Maxipreps in less than 20 minutes
ZymoPURE II is plasmid purification reinvented. At the core of our ZymoPURE II technology is a novel binding chemistry and membrane that redefines plasmid purity, reduces processing time by 7 fold, and enables > 1 mg of plasmid to be eluted from a microcentrifuge column.
Elution means Elution.
Tired of performing tedious alcohol precipitations? With ZymoPURE II, the plasmid DNA is bound onto a spin-column and all of the contaminants are washed away prior to eluting in a microcentrifuge. The resulting eluate is transfection-ready and highly concentrated, so there is no need to remove salts and concentrate the plasmid after elution. Be confident in your elution with ZymoPURE.
Good-bye Gravity Flow.
Say farewell to the drip. Your time is valuable, so why spend it waiting for gravity? With ZymoPURE II, the plasmid DNA is bound onto a spin-column in mere seconds using a vacuum manifold or centrifuge, which completely eliminates the need for slow gravity flow columns.
Elute from a Spin Column
Load on Vacuum
No Ethanol Precipitation
Tiny but mighty.
Minimum Elution Volume
Consistently elute ≥ 1 µg/µl
Highly Concentrated Eluates
Novel ZymoPURE II binding technology results in 6 times more DNA using 65 times less matrix.
Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate).
Plasmid DNA yield and concentration from the ZymoPure II Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
EndoZero. Demand Zero or Nothing.
Endotoxins below FDA Limit for Vaccines
Sensitive Transfection Ready
In Vivo Ready
ZymoPURE II has set the new standard for purity. Are you sure endotoxins are not affecting your results? Why take the risk? Eliminate any chance of adverse effects with a simple spin.
Redefine Purity with EndoZero Plasmid DNA
Stated manufacturers’ endotoxin levels for the ZymoPURE II Maxiprep kit compared to two separate kits from Supplier Q.
Confident and Consistent Handling
See it to believe it.
Visualize complete lysis and neutralization for easy, error free preparation.
Patented Multi-Colored Buffer System
The easy choice.
|ZymoPURE II Maxiprep Kit||Supplier Q Endofree Maxiprep||Supplier Q Maxiprep|
|Maximum Yield||1,200 µg||500 µg||500 µg|
|Endotoxins||≤ 0.025 EU/µg||≤ 0.1 EU/µg||≤ 10 EU/µg|
|Time per Prep||< 20 min||150 min||160 min|
|Hands-On Time||15 min||45 min||45 min|
|Load via Vacuum or Centrifuge (No Gravity Flow)|
|Elute from a Spin-Column (No Precipitation)|
|In Vivo & Gene Therapy Grade|
|Transfection Grade (Sensitive & Primary Cells)|
* Endotoxins below the FDA limit for Vaccines ( < 0.04 EU/µg of plasmid DNA)
Suitable for Any Application
Cited for sensitive techniques.
|Lentivirus Production||Adenovirus Production|
|Cell-Free Protein Synthesis||Generate Transgenic Organisms|
|Site-Directed Mutagenesis||Microinjection of Embryos|
|Cloning||In Vitro Transcription|
Highest Customer Satisfaction
"Overall, the kit is really easy to use, quick and the yield was the best I have ever obtained from a midi"
- H.H., MIT
“I love it. Easy to use, good yield. I WILL definitely replace my Q kit with this”
- K.J., Mayo Clinic School of Medicine
“It was very easy to use, taking hours off my prep time. The yield was good, 363 µg & 516 µg from 50 ml cultures, and the quality of DNA was also good. I wish I had more of these kits right now!”
- M.V. University of Pennsylvania
“This kit is exactly what our lab has been looking for”
- H.M., Washington University in St. Louis
“This kit is pretty great, midiprep DNA in about the time it takes to do a miniprep.”
- J.G., Yale University
Customer data demonstrates that isolated plasmid DNA is transfection-ready
Data generated by V.B. at University of Cologne. HeLa cells seeded in a 6-well plate were transfected with either 2 or 4 µg of pCI-neo® + GFP plasmid isolated from 100 ml of bacteria culture using the ZymoPURE Midiprep kit, ZymoPURE Maxiprep kit, or NucleoBond® Xtra Midi kit from Macherey-Nagel. GFP expression was assesed 48 hours later in cell lysates using western blot and Ponceau S staining. The blot was also probed with an antibody against alpha-Tubulin in order to verify equal loading of samples.
Plasmid Purification Reinvented
|Processing Volume||Processing Time||Yield||Elution Volume||Endotoxins||Instruction Manual|
|ZymoPURE Plasmid Miniprep Kit||≤ 5 ml||≤ 15 min||≤ 100 µg||≥ 25 µl||≤ 1 EU/µg DNA|
|ZymoPURE II Plasmid Midiprep Kit||≤ 50 ml||≤ 20 min||≤ 400 µg||≥ 100 µl||≤ 0.025 EU/µg DNA|
|ZymoPURE II Plasmid Maxiprep Kit||≤ 150 ml||≤ 20 min||≤ 1.2 mg||≥ 200 µl||≤ 0.025 EU/µg DNA|
|ZymoPURE II Plasmid Gigaprep Kit||≤ 2.5 L||≤ 50 min||≤ 10 mg||≥ 2 ml||≤ 0.025 EU/µg DNA|