ZymoPURE II - Plasmid Purification Made Simple

Manifold buffers

20 Minute Midi & Maxipreps

As quick as

20 min

Midi & Maxi preps

Up to

1.2 mg

from a spin-column

Up to

400x

less endotoxin

Plasmid Purification Reinvented

Plasma purification reinvented

Highest Yield. Lowest Elution Volume.

Highest yield, lowest elution volume

Transfection-grade Plasmid DNA

Transfection-grade Plasmid DNA
Transfection-grade Plasmid DNA 400x less endotoxin
400x

Less Endotoxin

Transfection-grade Plasmid DNA application ready
Application-ready

CRISPR, gene modification, lentiviral vectors, synthetic biology, etc.

Transfection-grade Plasmid DNA ultra-pure
Ultra-pure

For sensitive primary cells, in vivo injections, cloning, etc.

Plasmid Purity Redefined

Plasmid purity redefined

* Defined as endotoxins below the FDA limit for vaccines (< 0.04 EU/µg of plasmid DNA)

Colored Buffers Enable Easy, Error-free Preparation

Colored Buffers

Visualize alkaline lysis and neutralization steps using a patented multi-colored buffer system that enables easy determination of complete cell lysis and neutralization to prevent errors in processing.

Cited for Sensitive Applications

Cited for Sensitive Applications

Highest Customer Satisfaction

Highest Customer Satisfaction

Unrivaled Technology

ZymoPURE II Maxiprep Kit Supplier Q Endofree Maxiprep Supplier Q Maxiprep
Maximum Yield 1,200 µg 500 µg 500 µg
Endotoxins ≤ 0.025 EU/µg ≤ 0.1 EU/µg ≤ 10 EU/µg
Prep Time < 20 min 150 min 160 min
7 Min Bind, Wash, Elute
Spin Column Elution
Vaccine Grade*
Transfection Grade

* Defined as endotoxins below the FDA limit for vaccines (< 0.04 EU/µg of plasmid DNA)

ZymoPURE plasmid purification kits are the fastest and simplest plasmid purification methods available to efficiently isolate a high yield of transfection-grade plasmid DNA from E. coli in less than 20 minutes. Plasmid DNA is endotoxin-free and ready for immediate use in downstream applications such as transfection, in vivo injections, in vitro transcription, molecular cloning, PCR, and many more.

Generations Ahead Design

Generations Ahead Design

With this advanced plasmid purification spin-column technology, high yields of concentrated (up to 3 mg/ml), endotoxin-free plasmid DNA. The unique spin-column design included in this plasmid extraction kit also provides zero buffer retention and low elution volume.

ZymoPURE Plasmid Purification Kits

ZymoPURE Plasmid Miniprep Kit ZymoPURE II Plasmid Midiprep Kit ZymoPURE II Plasmid Maxiprep Kit ZymoPURE II Plasmid Gigaprep Kit
Processing Volume ≤ 5 ml ≤ 50 ml ≤ 150 ml ≤ 2.5 L
Processing Time ≤ 15 min ≤ 20 min ≤ 20 min ≤ 50 min
Yield ≤ 100 µg ≤ 400 µg ≤ 1.2 mg ≤ 10 mg
Elution Volume ≥ 25 µl ≥ 100 µl ≥ 200 µl ≥ 2 ml
Endotoxins ≤ 1 EU/µg DNA ≤ 0.025 EU/µg DNA ≤ 0.025 EU/µg DNA ≤ 0.025 EU/µg DNA
Instruction Manual

The ZymoPURE family of plasmid purification kit formats handle different amounts of culture input, ranging from miniprep (≤ 5 ml), midiprep (≤ 50 ml), maxiprep (≤ 150 ml) and gigaprep (≤ 2.5 L).

Try a Free Sample

Try a free sample

Up to

100 µg

from ≤ 5 ml culture

Miniprep

Get Free Sample

Up to

400 µg

from ≤ 50 ml culture

Midiprep

Get Free Sample

Up to

1200 µg

from ≤ 150 ml culture

Maxiprep

Get Free Sample

ZymoPURE Plasmid Purification Kits Product Description

The ZymoPURE plasmid purification kits are the best method for rapid isolation of transfection ready plasmid DNA. Plasmid purification for Mini preps, Midi preps and Maxi preps is performed in less than 20 minutes and Giga preps in 50 minutes. This family of plasmid purification kits feature a patented binding chemistry that enables simple purification of highly-concentrated (up to 3 mg/ml) endotoxin-free plasmid DNA on a spin-column. The streamlined workflow eliminates slow gravity flow anion-exchange columns and isopropanol precipitation steps found in other kits. ZymoPURE plasmid purification kits reduce processing time by up to 7x using a vacuum manifold or centrifugation. Simply bind, wash and elute transfection ready, endo-free plasmid in minutes.

The ZymoPURE plasmid purification kits are optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA, resulting in plasmids that are suitable for use in sensitive applications. ZymoPURE II plasmid purification kits include the EndoZero columns that enable rapid isolation of endotoxin-free plasmid DNA. The resulting endo-free plasmid DNA is ideal for transfection1 (including sensitive and primary cells), CRISPR-Cas9 and gene editing2, lentiviral vectors3, adenovirus vectors3 and AAV vectors3, gene therapy, chimeric antigen receptor (CAR) generation4, recombinant antibody generation5, in vitro transcription6, synthetic biology7, PCR8, transgenic organism generation9 and microinjections10, molecular cloning11, restriction endonuclease digestions, site-directed mutagenesis12, plasmid transformation of competent cells, Sanger sequencing13, and other sensitive downstream applications.

Applications of Use

  1. Transfection experiments (stable transfection and transient transfection) require highly-concentrated plasmid DNA that is free of salt contaminants and endotoxins otherwise transfection efficiency is reduced. An example of ZymoPURE used in transfections:
  2. Koblan, LW, et al., Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology, 843-846 (2018).

  3. Gene Editing techniques such as CRISPR-Cas9, require the delivery of endotoxin-free, concentrated vectors to ensure high efficiency of plasmid transfection. Examples of ZymoPURE used for gene editing:
  4. Findlay, GM, et al. Accurate classification of BRCA1 variants with saturation genome editing. Nature 562, 217-222 (2018).

    Hu, JH, et al. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature, 57-63 (2018).

    Salasova, A, et al. A proteomic analysis of LRRK2 binding partners reveals interactions with multiple signaling components of the WNT/PCP pathway. Molecular Neurodegeneration, 12(54) (2017).

  5. Lentivirus, Adenovirus and AAV vector production for use in transduction experiments, require up to four separate plasmids to be transfected into cells. Such a high number of expression plasmids requires purified plasmids that are highly concentrated and endo-free, to ensure high transfection efficiencies. Examples of ZymoPURE used for viral vector production:
  6. Jaitin, D.A, et al. Dissecting immune circuits by linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. Cell, 1883-1896 (2016).

    Salasova, A, et al. A proteomic analysis of LRRK2 binding partners reveals interactions with multiple signaling components of the WNT/PCP pathway. Molecular Neurodegeneration, 12(54) (2017).

    Chamberlain, K, et al. A Calsequestrin Cis-Regulatory Motif Coupled to a Cardiac Troponin T Promoter Improves Cardiac Adeno-Associated Virus Serotype 9 Transduction Specificity. Human Gene Therapy, 29(8) (2018).

  7. Chimeric antigen receptors (CAR) generation in T-cells requires highly concentrated, endotoxin-free, pure plasmids that are transfection-ready. An example of ZymoPURE used in T-cell receptor research:
  8. Takemasa, T, et al. Rapid Construction of Antitumor T-Cell Receptor Vectors from Frozen Tumors for Engineered T-Cell Therapy. Cancer Immunology Research 6(5) (2018).

  9. Recombinant Antibody Generation and expression uses transfection of highly concentrated plasmid DNA to prevent dilution of transfection reagents and loss of transfection efficiency. An example of ZymoPURE used in recombinant antibody production:
  10. Vazquez-Lombardi, R, et al. Transient expression of human antibodies in mammalian cells. Nature Protocols, 13(1), 99–117 (2017).

  11. In vitro transcription requires templates that are free of E. coli RNA, salts, proteins and other contaminants that can inhibit efficient transcript production. An example of ZymoPURE used for in vitro transcription:
  12. Marshall, R, et al. Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System. Molecular Cell, 146-157 (2017).

    Peñalber-Johnstone, C, et. al. Optimizing cell-free protein expression in CHO: Assessing small molecule mass transfer effects in various reactor configurations. Biotechnology and Bioengineering, 114(7), 1478–1486 (2017).

    Hunter, DJB, et al. Unexpected instabilities explain batch-to-batch variability in cell-free protein expression systems. Biotechnology and Bioengineering, 115(8), 1904–1914 (2018).

  13. Synthetic biology is a discipline that incorporates plasmid into workflows to engineer genomes and pathways to create, direct or manipulate desired functions. An example using ZymoPURE in a synthetic biology workflow to increase controlled protein production:
  14. Lillacci, G, et al. Synthetic control systems for high performance gene expression in mammalian cells. Nucleic Acids Research 46(18), 9855-9863 (2018).

  15. PCR using plasmid as DNA templates requires elimination of PCR-inhibitor contaminants found in purification methods, such as salts, detergents, nucleases and phenol. ZymoPURE plasmid purification yields that eliminates nucleases, detergents and salts, while using a phenol-free workflow.
  16. Transgenic organism generation uses endotoxin-free, purified plasmids in the process genetic engineering of target organisms using generating transgenes encoded on the plasmid. An example of ZymoPURE used to generate transgenic lines of D. melanogaster:
  17. Champer J, et al. Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. PLoS Genet 13(7), (2017).

  18. Microinjection of embryos require concentrated, endotoxin-free plasmid DNA to ensure the delivery of transgenes is clean and free of toxic contaminants. An example of ZymoPURE used in microinjection of C. elegans:
  19. Aram, R, et al. Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes. microPublication Biology (2019).

  20. Molecular cloning is the process of incorporating genes or other DNA sequences into plasmids. Whether isolating plasmid from E. coli for a restriction enzyme digest, purifying an entry clone for Gateway cloning, or purifying recombined plasmid from the Gibson cloning reagent mixture, highly pure plasmid DNA is required that is free from contaminants that can inhibit enzymatic reactions.
  21. An example of ZymoPURE purified plasmid after Gibson assembly:

    Lillacci, G, et al. Synthetic control systems for high performance gene expression in mammalian cells. Nucleic Acids Research, 46 (18), 9855-9863 (2018).

    An example of ZymoPURE purified plasmid after Gateway cloning:

    Xiong, J, et al. Development of a Time-Resolved Fluorescence Resonance Energy Transfer Ultrahigh-Throughput Screening Assay for Targeting the NSD3 and MYC Interaction. Assay and Drug Development Technologies 16(2), 96-106 (2018).

  22. Site-directed mutagenesis relies on ultra-pure, high quality plasmid DNA that is free from PCR inhibitors. An example using ZymoPURE after site-directed mutagenesis:
  23. Conway, JM, et al. Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species. AIChE Journal, 64(12) (2018).

  24. Sanger sequencing requires purified plasmid to be of high-quality to prevent contaminants from interfere with the efficiency of the sequencing reaction. An example using ZymoPURE prior to plasmid sequencing:
  25. McCullock, TW, et al. Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen. PLoS ONE, 15(2) (2020).