Protein & Enzymes
Overview - Protein & Enzymes
Although the expression and purification of recombinant proteins in E. coli is now a routine procedure, high-level expression or over-expression is not always attainable. However, those at Zymo Research have designed products to exploit the fact that high levels of protein expression can be consistently obtained when the processes of cell expansion and protein expression are kept separate. This is easily achieved with the use of the Dual Media Set™ where the over-expression of many proteins can be reliably controlled. In conjunction with the Dual Media Set™, our XJ Autolysis™ expression strains are ideal hosts for recombinant protein expression. With these strains, bacterial cell lysis is complete after a single freeze/thaw cycle. Researchers will find the single step lysis procedure simple, reproducible, and faster than conventional methods.
Expressing recombinant proteins in E.coli is now a routine procedure, though varying levels of protein expression are attainable. Research has proven that high levels of protein expression can be obtained when cell expansion and protein expression are kept separate. The ‘Dual Media Set’ by Zymo Research Corporation takes advantage of this with its two specially prepared mediums. This allows protein overexpression to be easily controlled for many recombinant proteins.
Bacterial Expression Strains
Zymo Research Corporation has produced XJ Autolysis E.Coli Strains, as the ideal hosts for recombinant protein expression. These strains allow complete bacterial lysis after a single freeze/thaw cycle. This single step lysis provides researchers with a fast, reproducible and simple procedure, resulting in high levels of protein expression.
Tag-Spin Protein Purification
The purification of His-tagged proteins is invaluable to the success of downstream applications such as enzymatic assays, protein biochemical analyses and SDS-PAGE. Zymo Research Corporation has produced the ‘His-Spin Protein Miniprep,’ to provide researchers with a fast and easy to follow procedure for purifying His-tagged proteins. This kit also allows bacterial colonies to be screened directly on the basis of protein expression vs. plasmid DNA.
Restriction enzymes are an extremely helpful tool in a number of research applications. From molecular cloning to epigenetics, Zymo Research provides a number of restriction enzymes that recognize different cut sites, including methylated and hydroxymethylated modifications of cytosine bases.