Unbiased DNA and RNA extraction for microbiome profiling

Cartoon DNA
  • Easy and Quick Workflow
  • Bias-Free Nucleic Acid Extraction
  • Inhibitor-Free DNA/RNA

Quick and Easy Workflow

Bind, Wash, Elute.

Bead beat to bind wash elute
  • No precipitation
  • No ice bath
  • No heat steps
  • No enzymes
ZymoBIOMICS DNA/RNA kit purity graph
Sample to Pure DNA in <30 min

Quick and Easy Workflow:
Bind, Wash, Elute

ZymoBIOMICS kit workflow

A wide array of samples can be easily loaded into the ZR BashingBead Lysis Tubes for complete and unbiased lysis, followed by filtering the lysate for debris removal. Samples are then processed using our patented Zymo-Spin Technology, making DNA extraction as easy as Bind, Wash, and Elute. Additionally, this kit is equipped with Zymo Research’s proprietary OneStep PCR Inhibitor Removal technology enabling PCR from the most PCR prohibitive environmental samples.

Bias-Free Nucleic Acid Extraction

Built for Accuracy

ZymoBIOMICS extraction methods

Four different extraction methods were assessed using the defined ZymoBIOMICS Microbial Community Standard and 16S sequencing.

Unbiased Lysis of Samples

Microbial composition graph

The ZymoBIOMICS DNA Miniprep Kit provides unbiased representation of the organisms extracted from the ZymoBIOMICS Microbial Community Standard. DNA was extracted from ZymoBIOMICS Microbial Community Standard using four different DNA extraction methods (ZymoBIOMICS DNA Miniprep Kit, Human Microbiome Project Protocol, Supplier Q1, and Supplier Q2) and analyzed using 16S rRNA gene sequencing.

Reveal the True Composition of Samples

Graph showing DNA yield and Microbial composition

Total DNA from samples of human feces was extracted using the ZymoBIOMICS DNA Miniprep Kit, HMP fecal DNA extraction protocol, Supplier M and Supplier Q. The ZymoBIOMICS DNA Miniprep Kit yielded a higher total amount of DNA (upper). Additionally, the HMP, Supplier M and Supplier Q protocols over-represented easy-to-lyse microbes, e.g. Bacteroidetes and Proteobacteria, biasing the microbial profile.

Inhibitor-Free DNA/RNA

Next-Gen sequencing ready.

ZymoSpin III-HRC

Certified Low Bioburden

Kits shouldn't bring their own microbes.

Certified Ultra-Clean certificate

Low Bioburden Certification

Bioburden graph

The bioburden of the kit was tested using real-time PCR with general bacterial primers. E.coli genomic DNA was used to create the standard curve.

High-Throughput Automation

Scale-up Analyses

Pipetting green liquid

Extract DNA from 96 Samples in ~1 Hour

Reproducible Sample Processing

Reproducibility graph

When 36 replicates of 200 µl blood samples from the same donor are processed, consistent and accurate yields are recovered in every sample. Average A260/230 ≥ 1.8.

No Cross-Contamination

Cross contamination graph

DNA is free from cross-contamination when purified with a liquid handler across a 96-well plate. The plate was set up with alternating rows of E. coli bacterial cells, HeLa cells, and S. cerevisiae yeast cells then simultaneously purified. Samples were evaluated using qPCR in duplicates

Product Catalog # Format Binding Capacity Protocol
ZymoBIOMICS DNA Miniprep Kit D4300 Spin Column 25 µg instruction manual  image
ZymoBIOMICS DNA Microprep Kit D4301 Spin Column 5 µg instruction manual image
ZymoBIOMICS-96 Magbead DNA Kit D4302 96 Magbead 10 µg instruction manual image
ZymoBIOMICS-96 DNA Kit D4309 96-Well Spin Column 5 µg instruction manual image
ZymoBIOMICS DNA/RNA Miniprep Kit R2002 Spin Column 100µg instruction manual image

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