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ZymoBIOMICS DNA/RNA Miniprep Kit

R2002


ZymoBIOMICS DNA/RNA Miniprep Kit

R2002

Cat # Name Size Price Quantity
R2002 ZymoBIOMICS DNA/RNA Miniprep Kit 50 Preps €545,00
€408,75
+ -

Documents


Purification of microbial DNA/RNA for microbiome or metagenome analyses

Highlights


  • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
  • Ultra-Pure: DNA and Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
  • High Sensitivity: Increased detection limit of very low abundance organisms.

Description


The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.

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Technical Specifications


Equipment Microcentrifuge, vortex, cell disrupter (recommended)
Purity DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range DNA and Total RNA ≥17 nt
Yield 100 µg DNA/RNA (binding capacity), ≥50 µl (elution volume)

Product Video


Citations


DNA and RNA were coextracted from human and mouse stomach biopsy using the ZymoBIOMICS DNA/RNA kit for downstream 16S rRNA sequencing targeting the hypervariable region V4. Microbiota analysis revealed Firmicutes being more transcriptionally active than Bacteroidetes along with selective enrichment and depletion of specific bacteria in the stomach.

Wurm, P et al. Qualitative and Quantitative DNA- and RNA-Based Analysis of the Bacterial Stomach Microbiota in Human, Mice, and Gerbils. mSystems. 2018.

Researchers used 16s rRNA gene sequencing to identify the microbial community in a sulfur-rich, low-temperature environment and showed not only the presence of sulfur oxidizing microbes but also aerobic Flavobacterium. The DNA was extracted from mineral precipitate using ZymoBIOMICS DNA/RNA.

Trivedi, CB et al. Low-Temperature Sulfidic-Ice Microbial Communities, Borup Fiord Pass, Canadian High Artice. Frontiers in Microbiology. 2018.

The ZymoBIOMICS DNA/RNA kit was used for co-extraction from 0.22 μm nitrocellulose water filters preserved in DNA/RNA Shield as well as biofilm membranes. Metagenomic and metatranscriptomic sequencing showed decreased microbial diversity and load after microfiltration and reverse osmosis. Water treatment also reduced the presence of antibiotic resistance genes and viruses.

Stamps, BW et al. Characterization of the Microbiome at the World’s Largest Potable Water Reuse Facility. Frontiers in Microbiology. 2018.

Kit Components