ZymoBIOMICS DNA Microprep Kit

D4301 / D4305


ZymoBIOMICS DNA Microprep Kit

Cat # Name Size Price Quantity
D4301 ZymoBIOMICS DNA Microprep Kit (Lysis Tubes) 50 Preps €274,00
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D4305 ZymoBIOMICS DNA Microprep Kit (No Lysis Matrix) 50 Preps €202,00
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ZymoBIOMICS DNA Microprep Kit

Highlights
  • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Description

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

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Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water
Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
Purity High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Supplemental Info
Type Total DNA
Yield Up to 5 µg total DNA can be eluted into ≥ 10 µl
Cat # Name Size Price
C1059-50 Zymo-Spin II-µHRC Filters 50 Pack €108,00
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes €101,00
D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml €98,00
D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml €32,00
D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml €46,00
D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml €138,00
D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml €40,00
C1057-50 Zymo-Spin III-F Filters 50 Pack €62,00
C1004-50 Zymo-Spin IC Columns 50 Pack €55,00