ZymoBIOMICS DNA Microprep Kit

D4301 / D4305


ZymoBIOMICS DNA Microprep Kit

Cat # Name Size Price Quantity
D4301 ZymoBIOMICS DNA Microprep Kit (Lysis Tubes) 50 Preps €306,80
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D4305 ZymoBIOMICS DNA Microprep Kit (No Lysis Matrix) 50 Preps €226,20
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Documents

ZymoBIOMICS DNA Microprep Kit

Highlights
  • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Description

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

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Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water
Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
Purity High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Supplemental Info
Type Total DNA
Yield Up to 5 µg total DNA can be eluted into ≥ 10 µl

Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).

Check sample type and input amount. Low biomass samples such as swabs, some soil samples, water, air are expected to have low yield. High biomass samples such as feces may cause overloading of the spin column. Using less input may improve yields.

Ensure sample is fully homogenized. Depending on bead beater used, the settings may have to be optimized for each sample. The homogenization time may be extended to see if yields improve.

Apply heated elution buffer (60-70° C) and allow elution buffer to incubate on the column for several minutes prior to elution.

Ensure that the DNA Elution Buffer is added directly to the column matrix.

Low A260/230 values can be due to handling issues (e.g. transfer of column during wash steps). To minimize reagent wash buffer carryover, centrifuge the column at max speed for 1 minute prior to elution.

Spin down lysate and Binding Buffer mixture at max speed for 5 minutes, this will eliminate any debris not caught by Spin III-F filter. Also avoid debris in lysis tube when transferring lysate, spin at max speed after bashing for 1-3 minutes.

We have validated our kits with both high-speed homogenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficient (do not use adapters made of foam). You can reference the Optimized Lysis Protocols table under documents for some recommendations.

This is to remove any debris from the lysate that could interfere with the DNA binding to the silica membrane of the column.

Cat # Name Size Price
C1059-50 Zymo-Spin II-µHRC Filters 50 Pack €121,00
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes €113,10
D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml €109,80
D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml €35,90
D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml €51,50
D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml €154,50
D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml €44,80
C1057-50 Zymo-Spin III-F Filters 50 Pack €66,10
C1004-50 Zymo-Spin IC Columns 50 Pack €59,40