ZymoBIOMICS DNA Microprep Kit
D4301 / D4305

Highlights
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Description
Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
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Elution Volume | ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water |
Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
Supplemental Info | |
Type | Total DNA |
Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl |
Cat # | Name | Size | Price | |
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C1059-50 | Zymo-Spin II-µHRC Filters | 50 Pack | €108,00 | |
S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | €101,00 | |
D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml | €98,00 | |
D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml | €32,00 | |
D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml | €46,00 | |
D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml | €138,00 | |
D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml | €40,00 | |
C1057-50 | Zymo-Spin III-F Filters | 50 Pack | €62,00 | |
C1004-50 | Zymo-Spin IC Columns | 50 Pack | €55,00 | |