Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
Description
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. |
| Size Range | Total RNA ≥17 nt |
| Supplemental Info | |
| Yield | 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
Q1: What is the purpose of the Zymo-Spin III-HRC Step?
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA/RNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).
Q2: What do I do if I notice precipitation during ethanol addition?
Sample overloading can cause nucleic acids to precipitate and crash out of solution, reducing yields. Use less sample input to avoid precipitation.
Q3: What do I do if there is foaming of sample during homogenization?
This is a normal occurrence as DNA/RNA Shield contains detergents. To reduce foam, centrifuge at 12,000 x g in 1 minute intervals before transferring the supernatant.
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml | €109,80 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | €24,70 | |
| R1100-50 | DNA/RNA Shield | 50 ml | €69,30 | |
| R1003-3-24 | RNA Wash Buffer | 24 ml | €66,10 | |
| R1060-1-50 | RNA Lysis Buffer | 50 ml | €88,50 | |
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | €61,60 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | €44,80 | |
| C1001-50 | Collection Tubes | 50 Pack | €16,80 | |
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack | €121,00 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | €16,80 | |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | €113,10 | |