Quick-DNA/RNA FFPE Miniprep Kit


Quick-DNA/RNA FFPE Miniprep Kit

Cat # Name Size Price Quantity
R1009 Quick-DNA/RNA FFPE Miniprep Kit 50 Preps €350,00
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Documents

DNA and RNA from FFPE tissue

Highlights
  • Easy Processing: Included Deparaffinization Solution for simple paraffin removal. No xylene necessary.
  • Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery.
  • High Quality: Non-crosslinked DNA and DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
Description

The Quick-DNA/RNA™ FFPE Kits provide a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free­ and is ready for RT-PCR, hybridization, sequencing, etc.

Equipment Microcentrifuge, vortex, and heat block/bath
Purity DNA/RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: ≥1.8.
Sample Source  Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
Size Range DNA and Total RNA ≥17 nt
Yield 50 µg DNA/RNA (binding capacity), ≥25 µl (elution volume)
Cat # Name Size Price
E1010-1-4 DNA Digestion Buffer 4 mL €15,00
D3050-1-5 2X Digestion Buffer - 5 mL 5 mL €10,00
D7010-3-24 DNA/RNA Wash Buffer (Concentrate) 24 ml €60,00
D7001-1-50 DNA/RNA Lysis Buffer 50 ml €79,00
D7010-2-50 DNA/RNA Prep Buffer 50 ml €63,00
C1001-50 Collection Tubes 50 Pack €16,00
C1011-50 Zymo-Spin IIC Columns 50 Pack €58,00
W1001-30 DNase/RNase-Free Water 30 ml €22,00
D3067-1-20 Deparaffinization Solution 20 ml €22,00