Quick-DNA/RNA FFPE Miniprep Kit
- Easy Processing: Included Deparaffinization Solution for simple paraffin removal. No xylene necessary.
- Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery.
- High Quality: Non-crosslinked DNA and DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
The Quick-DNA/RNA™ FFPE Kits provide a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
|Equipment||Microcentrifuge, vortex, and heat block/bath|
|Purity||DNA/RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: ≥1.8.|
|Sample Source||Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2|
|Size Range||DNA and Total RNA ≥17 nt|
|Yield||50 µg DNA/RNA (binding capacity), ≥25 µl (elution volume)|