Quest 5-hmC DNA ELISA Kit
D5425 / D5426
- Sensitive and specific detection of 5-hydroxymethlycytosine (5-hmC) from a variety of samples
- Ideal for global 5-hmC quantitation and high throughput screening
- Streamlined workflow can be completed in 3 hrs
The Quest 5-hmC DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments. The Control DNA Set included with this kit has been calibrated to accurately quantify the percent 5-hmC in sample DNA by use of a standard curve. Also, the fast, streamlined workflow is ideal when analyzing/screening large numbers of samples.
|Detection||≥ 0.02% 5-hmC per 100 ng DNA|
|Equipment||Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.|
|Input||This protocol is optimized for 100 ng of input DNA/well. Compatible with DNA in the range of 20-200 ng/well.|
|Sample Source||Purified genomic DNA in water, Tris-EDTA, or similar.|
Q1: What is the shelf life of the ELISA kits?
Q2: How to store the components of the 5h-mC ELISA kit?
Q3: Where are the Control DNA derived from?
Q4: Which wavelength should be used for the ELISA?
Q5: How to quantify the percentage of 5-hmC in a DNA sample?
Q6: Why is there no color development after addition of HRP developer?
Q7: My ODs so low. What should I check?
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