OneStep qMethyl-PCR Kit

D5310 / D5311


OneStep qMethyl-PCR Kit

D5310 / D5311

Cat # Name Size Price Quantity
D5310 OneStep qMethyl-PCR Kit Full Kit €399,00
+ -
D5311 OneStep qMethyl-Lite Lite €369,00
+ -

Documents


OneStep qMethyl-PCR Kit

Highlights


  • Single step, real-time PCR procedure for bisulfite-free determination of DNA methylation status
  • Includes reagents and controls for quantitative detection and reliable performance
  • Ideal for rapid screening of single and multi-locus DNA methylation

Description


The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation.

Technical Specifications


Input High quality DNA suitable for use in restriction enzyme digestion. Require A260/280 g&t; 1.8, A260/230 > 1.8.
Processing Time 4.5 hours
Storage Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C. 2X Test Reaction PreMix and 2X Reference Reaction PreMix are light sensitive. Minimize light exposure.

Citations


Accurate DNA methylation detection using the OneStep qMethyl Kit was used to assess the percent of DNA methylation in the promoter region of two potential epigenetic biomarkers for pregnancy-related diseases. The DNA methylation detection was able to correlate the increase in DNA methylation with the increase in cellular invasion using DNA samples from first trimester placentas that were known to have an outgrowth potential.

van Dijk M et al. (2012) Naturally occurring variation in trophoblast invasion as a source of novel (epigenetic) biomarkers. Front Genet.; 3:22.

Kit Components