Methylated-DNA IP Kit
- Robust enrichment & immunoprecipitation of DNA containing 5mC.
- Includes a highly specific anti-5-methylcytosine monoclonal antibody for defined, reproducible results.
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses (e.g., assembling genomic libraries and determining genome-wide methylation status).
|Applicable For||Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc.|
|Elution Volume||≥ 15 µl|
|Input||50 - 500 ng of DNA|
|Processing Time||4 hours|
Methylated DNA was enriched from paired-end libraries generated by the authors using the Methylated-DNA IP Kit from Zymo Research and then sequenced using the Illumina HiSeq. The results showed that MeDIP-Seq detected twice as many differentially methylated regions than the Illumina 450k array method.Clark, C. et al. (2012) A comparison of the whole genome approach of MeDIP-Seq to the targeted approach of the Infinium HumanMethylation450 BeadChip for methylome profiling. PLoS One 7(11): e50233.
Researchers used the Methylated DNA IP Kit from Zymo Research to immunoprecipitate methylated DNA from leaf tissue and study the DNA methylation profile in the maize genome. The authors found that specific families of retrotransposons could spread heterochromatin to neighboring genes and epigenetically suppress their expression.Eichten SR et al. (2012) Spreading of heterochromatin is limited to specific families of maize retrotransposons. PLoS Genet. 8(12):e1003127.
The Methylated-DNA IP Kit was used for CpG immunoprecipitation of genomic DNA isolated from T cells that was sheared to ~200 bp using dsDNA Shearase Plus (E2018) to study the DNA methylation status of the Notch-1 promoter.Rauen T et al. (2012) AMP-responsive element modulator α (CREMα) contributes to decreased Notch-1 expression in T cells from patients with active systemic lupus erythematosus (SLE). J Biol Chem. 287(51):42525-32.