Zymo Environ Water RNA Kit

Product image of the Zymo Environ Water RNA Kit

SARS-CoV-2 Surveillance
Made Simple

Streamlined Filter-Free Workflow

Common Challenges for Wastewater Viral RNA Extraction Zymo Environ Water RNA Kit
Large Sample Volumes Enhanced viral enrichment; get 8x more viral RNA
Infectious Pathogens Pathogen inactivation for safe handling
PCR Inhibition PCR inhibitors removed in one spin
Dilute Yields ≥ 6 µl elution improves limits of detection
Wastewater Workflow

Get 8x More SARS-CoV-2 RNA from Wastewater

The Zymo Environ Water RNA Kit includes Water Concentrating Buffer (WCB). 5 ml of SARS-CoV-2 positive wastewater was processed with and without WCB (n=2). RT-qPCR showed the addition of WCB shifted average Ct values earlier by ~3 (an 8-fold increase in viral recovery).

PCR Inhibitor-Free RNA

Extracted Wastewater RNA is free of PCR Inhibitors. 50K HeLa cells were spiked into DNA/RNA Shield™ (positive extraction control) and into wastewater. RNA was extracted (n=2) and RT-qPCR (Quasar 670) analysis showed the ΔCt is <0.5, indicating the extraction system effectively removes inhibitors in wastewater.

Detect Down to 8 Viral Copies

Improved Limit of Detection (LOD). SARS-CoV-2 sample was serially diluted from 5000 to 8 viral copies and spiked into proxy wastewater. RNA was extracted (n=2) using the Zymo Environ Water RNA Kit. RT-qPCR targeting the N1 gene showed the dilution is linear (R2 = 0.9898) and the workflow was able to achieve an LOD of 8-40 copies.

Safe Sample Inactivation

DNA/RNA Shield abides by Centers for Disease Control's (CDC) guidelines for pathogen inactivation.

Used by Scientists around the world for studying:

Leading Technology

Zymo Environ Water RNA Kit Other Suppliers
Enrichment Method Transforms ≤ 5 mL wastewater into a small pellet by centrifugation Requires filters (sold separately) or separate concentration method
Sample Inactivation DNA/RNA Shield inactivates infectious agents for safe handling Pathogens not inactivated
Elution Volume ≥ 6 µl 50-100 µl
Viral Sensitivity High RNA recovery and low elution volume enables a limit of detection (LOD) of 8 copies/sample Not optimized