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RNA Clean & Concentrator-5

R1013 / R1014 / R1015 / R1016


RNA Clean & Concentrator-5

R1013 / R1014 / R1015 / R1016

Cat # Name Size Price Quantity
R1013 RNA Clean & Concentrator-5 (DNase Included) 50 Preps €170,00
€127,50
+ -
R1014 RNA Clean & Concentrator-5 (DNase Included) 200 Preps €585,00
€438,75
+ -
R1015 RNA Clean & Concentrator-5 (DNase not Included) 50 Preps €142,00
€106,50
+ -
R1016 RNA Clean & Concentrator-5 (DNase not Included) 200 Preps €485,00
€363,75
+ -

Documents


RNA clean-up from any reaction

Highlights


  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
  • Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
  • Maximum Recovery: Recover >90% and elute in as little as 6 μl.

Description


The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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Technical Specifications


Equipment Microcentrifuge
Format Spin-Column
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥ 6 µl (elution volume)

Product FAQ


Q1: Can the RCC kit be used to purify DNA (cDNA)?

Q2: Should carrier RNA be added to the sample prior to purification?

Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?

Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?

Q6: Is DNase I treatment necessary?

Product Video


Citations


The RNA Clean & Concentrator™-5 was used to clean sgRNA and in vitro synthesized Cas9-encoding mRNA. The mRNA was injected in one-cell stage zebrafish embryo for a reverse genetic screening approach using CRISPR. Two new genes involved in electrical-synapse formation were identified by deep sequencing.

Shah AN, et. al. (2015). Rapid reverse genetic screening using CRISPR in zebrafish. Nature Methods. 12(6): 535-40.

We used the RNA Clean & Concentrator columns during our high-throughput sequencing library preparation. We used them to remove enzymes and buffers between each enzymatic reaction. We also used the >200nt protocol to remove unligated cloning linkers.

Talkish J, May G, Lin Y, Woolford JL Jr, & McManus CJ (2014). Mod-seq: high-throughput sequencing for chemical probing of RNA structure

Reviews


“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”

- Anupma C.

“The Zymo-Spin IC column is convenient.”

- Lihua D. (National Institutes of Health)

“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”

- Caroline S. (Cornell University)

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Kit Components