RNA Clean & Concentrator-25

R1017 / R1018


RNA Clean & Concentrator-25

R1017 / R1018

Cat # Name Size Price Quantity
R1017 RNA Clean & Concentrator-25 50 €142,00
+ -
R1018 RNA Clean & Concentrator-25 100 €254,00
+ -

Documents


RNA clean-up from any reaction

Highlights


  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
  • Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
  • Maximum Recovery: Recover >90% and elute in as little as 25 μl.

Description


The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 25 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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Technical Specifications


Equipment Microcentrifuge
Format Spin-Column
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size Range Total RNA ≥ 17 nt
Yield 50 µg RNA (binding capacity), ≥ 25 µl (elution volume)

Product FAQ


Q1: Can the RCC kit be used to purify DNA (cDNA)?

Q2: Should carrier RNA be added to the sample prior to purification?

Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?

Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?

Q6: Is DNase I treatment necessary?

Product Video


Citations


In experiments designed to investigate RNase E-mediated degradation of mRNAs in cyanobacteria, the RNA Clean & Concentrator-25 was used to both concentrate gel-purified in vitro transcribed RNA as well as to clean up total cellular RNA for reverse transcription following DNase treatment. The researchers found that regulatory antisense RNAs are able to protect mRNA from being degraded by RNase E through forming RNA-RNA complexes with their target mRNA molecules.

Stazic et al. (2011) Antisense RNA protects mRNA from RNase E degradation by RNA–RNA duplex formation during phage infection. Nucleic Acids Res. 39(11), 4890–4899.

Reviews


"Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."

- Catherine A. (USAMRIID)

“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”

- Caroline S. (Cornell University)

"The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns."

- Agnes N. (Biofidal)

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Kit Components