Successfully Added to Cart
Customers also bought...
-
DNA/RNA Shield (50 ml)Cat#: R1100-50DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the...
-
DNA/RNA Shield SafeCollect Swab Collection Kit, 1ml (CE-IVD) (1 collection kit)Cat#: R1160-EThe DNA/RNA Shield SafeCollect Swab Collection Kit is a user-friendly collection kit for stabilizing the nucleic acid content of samples collected with a swab. DNA/RNA Shield completely inactivates harmful pathogens...
Quick-DNA/RNA Viral Kit
D7020 / D7021
Quick-DNA/RNA Viral Kit
Cat # | Name | Size | Price | Quantity |
---|
Documents

Highlights
- Quick recovery of viral DNA/RNA from plasma, serum and other samples.
- Omits the use of organic denaturants and proteases.
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
Description
Equipment | Microcentrifuge |
---|---|
Purity | High-quality nucleic acids are ready for Next-Gen Sequencing, RT/qPCR, hybridization, etc |
Sample Source | Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples |
Size Range | 50 nt to ~200 kb |
Supplemental Info | |
Yield | 25 µg DNA and 50 µg RNA |
Q1: Is the addition of beta-mercaptoethanol necessary?
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Q2: Is this kit compatible with samples stored in UTM/VTM?
Yes, these samples are compatible.
Q3: Will this kit isolate DNA?
Yes, this kit will co-purify some DNA.
Q4: Can DNase I treatment be performed?
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
Q7: Why are my columns/plate wells getting clogged?
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
Q8: Why am I seeing delayed/no amplification during RT/qPCR?
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
Cat # | Name | Size | Price | |
---|---|---|---|---|
C1102-25 | Zymo-Spin IIC-XL Columns | 25 Pack | €39,60 | |
C1102-50 | Zymo-Spin IIC-XL Columns | 50 Pack | €76,90 | |
D7020-1-25 | Viral DNA/RNA Buffer | 25 ml | €66,10 | |
D7020-1-100 | Viral DNA/RNA Buffer | 100 ml | €182,50 | |
R1034-2-6 | Viral Wash Buffer (concentrate) | 6 ml | €20,20 | |
C1001-50 | Collection Tubes | 50 Pack | €16,80 | |
R1034-2-24 | Viral Wash Buffer (concentrate) | 24 ml | €67,20 | |
W1001-6 | DNase/RNase-Free Water | 6 ml | €16,80 | |
W1001-1 | DNase/RNase-Free Water | 1 ml | €11,20 | |
R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | €245,30 | |