Quick-DNA/RNA Viral MagBead
R2140 / R2141 / R2140-E / R2141-E
Quick-DNA/RNA Viral MagBead
- High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples
- High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
- DNA/RNA Shield included for sample collection, inactivation and storage.
Quick-DNA/RNA Viral MagBead kit is designed for high-throughput purification of viral DNA and/or RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies and swab and fecal samples stored in DNA/RNA Shield. The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (>50 nt) and large (>200 kb) DNA and RNA are bound to magnetic beads, washed and eluted. The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.
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|Equipment||Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.|
|Purity||RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.|
|Sample Source||Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies and swab and fecal samples|
|Size Range||50 nt to ~200 kb|
|Yield||5 µg DNA/RNA binding capacity (per 10 µl MagBinding Beads), ≥15 µl elution volume.|
Q1: Is the addition of beta-mercaptoethanol necessary?
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Q2: Is this kit compatible with samples stored in UTM/VTM?
Yes, these samples are compatible.
Q3: Will this kit isolate DNA?
Yes, this kit will co-purify some DNA.
Q4: Can DNase I treatment be performed?
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
Q7: Why are my columns/plate wells getting clogged?
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
Q8: Why am I seeing delayed/no amplification during RT/qPCR?
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
|R1200-125||DNA/RNA Shield (2X Concentrate)||125 ml||€245,30|
|D3001-2-20||Proteinase K w/ Storage Buffer Set||20 mg||€49,30|
|D4100-2-12||MagBinding Beads||12 ml||€163,80|
|D4100-2-3||MagBinding Beads||3 ml||€86,50|
|W1001-30||DNase/RNase-Free Water||30 ml||€24,70|
|D7020-1-100||Viral DNA/RNA Buffer||100 ml||€182,50|
|R2130-1-120||MagBead DNA/RNA Wash 1||120 ml||€221,70|
|R2130-1-30||MagBead DNA/RNA Wash 1||30 ml||€70,60|
|R2130-2-20||MagBead DNA/RNA Wash 2||20 ml||€60,50|
|R2130-2-80||MagBead DNA/RNA Wash 2||80 ml||€191,50|