Quick-DNA/RNA Miniprep

D7001


Quick-DNA/RNA Miniprep

D7001

Cat # Name Size Price Quantity
D7001 Quick-DNA/RNA Miniprep 50 Preps €325,00
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Documents


Isolate DNA and RNA from any sample

Highlights


  • Quick & Easy: Extract DNA and RNA from cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood.
  • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)

Description


The Quick-DNA/RNA Miniprep Kit provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes.

Technical Specifications


Equipment Microcentrifuge, vortex
Purity DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood.
Size Range Genomic DNA ≥40 kb and Total RNA ≥17 nt
Yield 25 µg DNA and RNA (binding capacity), ≥25 µl (elution volume)

Product FAQ


Q1: What is the difference between the Quick-DNA/RNA Miniprep (D7001) and Quick-DNA/RNA Miniprep Plus (D7003)?

Q2: Is DNase I available for individual purchase?

Q3: Can I use other DNase I enzymes (or sets)? (e.g. Qiagen DNase I)?

Q4: How to store DNase-I following resuspension?

Q5: Is Quick-DNA/RNA suitable for very small numbers of cells?

Q6: Is the kit compatible with blood samples stored in PAXgene Blood Tubes?

Q7: Is the DNase-I treatment necessary?

Q8: How to purify RNA from samples stored in RNAlater?

Q9: Is it possible to extract proteins with the Quick-DNA/RNA kit?

Q10: I ran out of DNA/RNA Wash Buffer. Can I use something else?

Q11: Is the DNA and RNA suitable for Next-Gen Sequencing or other sensitive downstream applications?

Q12: Will the kit isolate small RNAs?

Q13: Can samples be stored in DNA/RNA Lysis Buffer prior to processing?

Q14: Is the kit compatible with samples stored in DNA/RNA Shield?

Reviews


“This kit was invaluable. I was able to get at least 1 µg of RNA and several hundred nanograms of DNA from each sample - all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”

- Julie P. (CU Anschutz Medical Campus)

“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”

- Robert B. (Harvard University)

"The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq)."

- Muhammad A. (Northwestern University)

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Kit Components