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Quick-DNA/RNA Microprep Plus Kit
D7005 / D7005T
Quick-DNA/RNA Microprep Plus Kit
- Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
- Sensitive: Single cell-level recovery of DNA and RNA.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
The Quick-DNA/RNA Microprep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from a wide range of sample sources, including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality gDNA and total RNA, ready for any downstream application including reverse transcription, microarray, sequencing.
|Equipment||Microcentrifuge, vortex, and heat block/bath (55°C)|
|Purity||DNA and RNA are ready for Next-Gen Sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, and samples stored in DNA/RNA Shield, etc.|
|Size Range||Genomic DNA ≥40 kb and Total RNA ≥17 nt|
|Yield||≤ 5 µg DNA and ≤ 10 µg RNA|
Q1: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q2: Can I use other DNase I enzymes (or sets)? (e.g. Qiagen DNase I)?
The kit is optimized with Zymo Research's DNase, however DNase from other manufacturers can be used. Follow the respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post-extraction, then purify using the RNA Clean & Concentrator-5.
Q3: How to store DNase-I following resuspension?
Aliquot and store at – 20°C. Minimize multiple freeze thaw cycles. Lyophilized DNase I is stable at room temperature.
Q4: Is this kit suitable for very small numbers of cells?
Yes, the Quick-DNA/RNA MicroPrep Plus (#D7005) is capable of isolating from single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q6: Is it possible to extract proteins with the Quick-DNA/RNA kit?
Yes, proteins can be precipitated. Please refer to the protocol appendix.
Q7: I ran out of DNA/RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. DNA/RNA Wash Buffer is also sold separately.
Q8: Is the DNA and RNA suitable for Next-Gen sequencing or other sensitive downstream applications
Yes, both DNA and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, qPCR, etc.
Q9: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q10: Can I freeze my samples in DNA/RNA Lysis Buffer for later processing?
Yes, freeze samples at -80°C after samples are lysed/homogenized in DNA/RNA Lysis Buffer. Bring the sample to room temperature prior to RNA purification.
Q11: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 1 volume of DNA/RNA Lysis Buffer (1:1) and mix well. Proceed with DNA Purification.
Q12: How should I proceed with this kit if my sample is in RNAlater™?
Cells - Pellet by centrifugation at up to 5,000 x g and remove RNAlater™ (supernatant). Proceed to Sample Preparation, see protocol.
Tissue - Transfer into a new tube with forceps and remove any excess RNAlater™. Proceed to Sample Preparation, see protocol.
Alternatively, for liquid samples from which RNAlater™ cannot be removed, add 1 volume of nuclease-free water (or PBS) to 1 volume liquid sample (1:1) and mix. Then add 4 volumes DNA/RNA Lysis Buffer to 1 volume sample/water (or PBS) mixture (4:1). Mix again and proceed to Total RNA Purification, see protocol.
“This kit was invaluable. I was able to get at least 1 ug of RNA and several hundred nanograms of DNA from each sample - all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”
- Julie P. (CU Anschutz Medical Campus)
“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”
- Robert B. (Harvard University)
"The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq)."
- Muhammad A. (Northwestern University)
|R1200-25||DNA/RNA Shield (2X Concentrate)||25 ml||€69,30|
|W1001-10||DNase/RNase-Free Water||10 ml||€21,30|
|C1004-50||Zymo-Spin IC Columns||50 Pack||€59,40|
|R1200-1-5||PK Digestion Buffer||5 ml||€18,00|
|C1001-50||Collection Tubes||50 Pack||€16,80|
|D7010-2-50||DNA/RNA Prep Buffer||50 ml||€70,60|
|D7001-1-50||DNA/RNA Lysis Buffer||50 ml||€88,50|
|D7010-3-24||DNA/RNA Wash Buffer (Concentrate)||24 ml||€67,20|
|E1010-1-4||DNA Digestion Buffer||4 mL||€16,80|
|C1103-50||Zymo-Spin IC-XM Columns||50 Pack||€73,40|