Quick-DNA Microprep Kit
D3021 / D3020
- Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
- Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
- Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
|Elution Volume||≥10 µl|
|Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8|
|Sample Source||Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered|
|Workflow||Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.|
|Yield||Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood yields 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.|
Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q3: Can the Quick-DNA kit be used with bacterial samples?
Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?
Q5: Can Quick-DNA process crude lysates?
Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q7: Is it possible to add an RNase A treatment to the protocol?
Q8: What are the expected yields for each sample type?
“It was easy to work with, protocol easy to follow”
- Tinatin T.
“This kit did a good job of prepping clean genomic DNA.”
- Tara N. (United States Agricultural Research Service)
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