Quick-DNA Miniprep

D3024 / D3025


Quick-DNA Miniprep

D3024 / D3025

Cat # Name Size Price Quantity
D3024 Quick-DNA Miniprep 50 Preps €98,00
+ -
D3025 Quick-DNA Miniprep 200 Preps €320,00
+ -

Documents


Rapid purification of DNA from cells and whole blood.

Highlights


  • Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
  • Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
  • Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Description


The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Technical Specifications


Elution Volume ≥50 µl
Equipment Microcentrifuge, Vortex
Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
Sample Source Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered
Workflow Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.
Yield Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.

Product FAQ


Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?

Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?

Q3: Can the Quick-DNA kit be used with bacterial samples?

Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?

Q5: Can Quick-DNA process crude lysates?

Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?

Q7: Is it possible to add an RNase A treatment to the protocol?

Q8: What are the expected yields for each sample type?

Citations


The Quick-gDNA MiniPrep enabled the recovery of high quality DNA from blood pathogens for whole genome analysis of bacterium that cannot be cultured in vitro. The sequenced genome provided clues to the mechanism of pathogenicity.

do Nascimento NC, Guimaraes AM, Santos AP, SanMiguel PJ, Messick JB. Complete genome sequence of Mycoplasma haemocanis strain Illinois. J Bacteriol. 2012;194(6):1605-6.

The Quick-gDNA MiniPrep kit was used to extract inhibitor-free DNA from the blood of pigs infected with Mycoplasma suis. The purified DNA was used in the development of a qPCR assay that is highly sensitive and specific for M. suis detection and quantification.

Guimarares AM, et al. (2011) A quantitative TaqMan PCR assay for the detection of Mycoplasma suis. J Appl Microbiol. 111(2):417-25.

Reviews


“It was easy to work with, protocol easy to follow”

- Tinatin T.

“This kit did a good job of prepping clean genomic DNA.”

- Tara N. (United States Agricultural Research Service)

"This product was amazing! I've used the same type of kit (quick DNA extract) from Sigma and this was far more superior. I used the same amount of postnatal tissue as I would have for the Sigma kit, however the yield I obtained from Zymo was quite astounding considering the time of tissue digestion. Secondly, the gDNA was much 'cleaner' upon measurement with our NanoDrop! "

- Stephen C. (Johns Hopkins University School of Medicine)

Kit Components