Quick-DNA 96 Kit

D3012 / D3011 / D3010


Quick-DNA 96 Kit

D3012 / D3011 / D3010

Cat # Name Size Price Quantity
D3012 Quick-DNA 96 Kit 10 x 96 Preps €860,00
+ -
D3011 Quick-DNA 96 Kit 4 x 96 Preps €402,00
+ -
D3010 Quick-DNA 96 Kit 2 x 96 Preps €214,00
+ -

Documents


Rapid purification of DNA from cells and whole blood

Highlights


  • Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
  • Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
  • Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Description


The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Technical Specifications


Elution Volume ≥ 25 µl
Equipment Microcentrifuge, vortex, centrifuge w/ microplate carriers
Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
Sample Source Whole blood, plasma, serum cultured cells, buccal cells, tissue already digested with Proteinase K or mechanically homogenized from human, mouse, rat, etc. are effectively processed using this kit.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered
Workflow Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.
Yield Up to 5 µg/well total DNA is eluted into ≥30 µl DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.

Product FAQ


Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?

Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?

Q3: Can the Quick-DNA kit be used with bacterial samples?

Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?

Q5: Can Quick-DNA process crude lysates?

Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?

Q7: Is it possible to add an RNase A treatment to the protocol?

Q8: What are the expected yields for each sample type?

Kit Components