ZR Plasmid Miniprep - Classic
D4054 / D4015 / D4016
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.|
|Elution Volume||≥ 30 µl|
|Endotoxin Levels||< 50 EU/µg|
|Processing Time||15 min|
|Purity||Typical Abs260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.|
Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?
Q2: Can the kit be used to isolate large constructs (BAC/PAC)?
Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?
Q4: What is the composition of the DNA Elution buffer?
Q5: Can I perform the procedure using a vacuum manifold?
Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?
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