ZR Plasmid Miniprep - Classic
D4054 / D4015 / D4016
ZR Plasmid Miniprep - Classic
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
|Applicable For||Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.|
|Elution Volume||≥ 30 µl|
|Endotoxin Levels||< 50 EU/µg|
|Processing Time||15 min|
|Purity||Typical Abs260/280 ≥1.8.|
|Size Range||Up to 25 kb|
|Yield||Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.|
Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?
The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.
Q2: Can the kit be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.
Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q4: What is the composition of the DNA Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q5: Can I perform the procedure using a vacuum manifold?
Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.
Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.
Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q8: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
|D3004-4-16||DNA Elution Buffer||16 ml||€20,20|
|D4036-3-60||Endo-Wash Buffer||60 ml||€38,10|
|D4036-3-30||Endo-Wash Buffer||30 ml||€20,20|
|D3004-4-4||DNA Elution Buffer||4 ml||€11,20|
|D4027-1-160||Buffer P1 (Red)||160 ml||€29,20|
|D4027-1-20||Buffer P1 (Red)||20 ml||€16,80|
|D4027-1-80||Buffer P1 (Red)||80 ml||€23,60|
|D4027-2-160||Buffer P2 (Green)||160 ml||€29,20|
|D4027-2-20||Buffer P2 (Green)||20 ml||€16,80|
|D4027-2-80||Buffer P2 (Green)||80 ml||€23,60|
|D4027-3-220||Buffer P3 (Yellow)||220 ml||€49,30|
|D4027-3-50||Buffer P3 (Yellow)||50 ml||€20,20|
|D4027-4-24||Plasmid Wash Buffer||24 ml||€39,20|
|D4027-4-48||Plasmid Wash Buffer||48 ml||€54,90|
|D4027-4-6||Plasmid Wash Buffer||6 ml||€11,20|
|C1001-50||Collection Tubes||50 Pack||€16,80|
|C1019-50||Zymo-Spin IIN Columns||50 Pack||€60,50|