ZR Plasmid Miniprep - Classic


ZR Plasmid Miniprep - Classic

Cat # Name Size Price Quantity
D4054 ZR Plasmid Miniprep - Classic 800 Preps €672,00
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D4015 ZR Plasmid Miniprep - Classic 100 Preps €109,00
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D4016 ZR Plasmid Miniprep - Classic 400 Preps €414,00
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Documents


Easy traditional method for isolating high-quality plasmid DNA from E. coli

Highlights

  • High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
  • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
  • Unique column design: Zero buffer retention and low (30 µl) elution volume.
Description

The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.


Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.
Elution Volume ≥ 30 µl
Endotoxin Levels < 50 EU/µg
Equipment Microcentrifuge
Processing Time 15 min
Purity Typical Abs260/280 ≥1.8.
Size Range Up to 25 kb
Yield Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.

Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?

The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.

Q2: Can the kit be used to isolate large constructs (BAC/PAC)?

No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.

Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?

Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

Q4: What is the composition of the DNA Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Q5: Can I perform the procedure using a vacuum manifold?

Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.

Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.

Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?

≤ 50 Endotoxin Units/µg of plasmid DNA.



Cat # Name Size Price
D3004-4-16 DNA Elution Buffer 16 ml €19,00
D4036-3-60 Endo-Wash Buffer 60 ml €36,00
D4036-3-30 Endo-Wash Buffer 30 ml €18,00
D3004-4-4 DNA Elution Buffer 4 ml €12,00
D4027-1-160 Buffer P1 (Red) 160 ml €26,00
D4027-1-20 Buffer P1 (Red) 20 ml €15,00
D4027-1-80 Buffer P1 (Red) 80 ml €21,00
D4027-2-160 Buffer P2 (Green) 160 ml €26,00
D4027-2-20 Buffer P2 (Green) 20 ml €15,00
D4027-2-80 Buffer P2 (Green) 80 ml €21,00
D4027-3-220 Buffer P3 (Yellow) 220 ml €44,00
D4027-3-50 Buffer P3 (Yellow) 50 ml €18,00
D4027-4-24 Plasmid Wash Buffer 24 ml €35,00
D4027-4-48 Plasmid Wash Buffer 48 ml €49,00
D4027-4-6 Plasmid Wash Buffer 6 ml €10,00
C1001-50 Collection Tubes 50 Pack €16,00
C1019-50 Zymo-Spin IIN Columns 50 Pack €57,00