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Quick-16S Plus NGS Library Prep Kit (V1-V3)
Cat # | Name | Size | Price | Quantity |
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Highlights
- The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
- 100% automation ready with only a single PCR step and without the need for normalization.
- Real-time PCR enables absolute microbial copy number quantification.
Documents
Product Description
Technical Specifications
16S V1-V3 Primer Sequences (adapters not included) | 27f (AGRGTTYGATYMTGGCTCAG, 20 bp) and 515r (TBACCGCGGCTGCTGGCAC, 19 bp). |
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Amplicon Size | The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~492 bp. |
Barcode Sequences | 10 bp barcodes are available to download here or by visiting the Documentation section of this Product Page. |
Index Primers | Unique dual index (barcodes) to label individual samples. |
Required Equipment | Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates. |
Sample Input | Purified microbial DNA ≤100 ng, free of PCR inhibitors. |
Sequencing Platform | Illumina MiSeq® or NextSeq 1000/2000® without the need to add custom sequencing primers. We recommend the MiSeq® Reagent Kit v3 (600-cycle) or NextSeq 1000/2000® Reagent Kit P1/P2 (600-cycle). For assistance with sample sheet setup, see Appendix F. |
Resources
Cat # | Name | Size | Price | |
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D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml | €32,30 | |
D6305 | ZymoBIOMICS Microbial Community DNA Standard | 200ng | €119,90 | |
D6306 | ZymoBIOMICS Microbial Community DNA Standard | 2000ng | €239,90 | |
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