EZ DNA Methylation-Lightning Automation Kit
D5049
EZ DNA Methylation-Lightning Automation Kit
Cat # | Name | Size | Price | Quantity |
---|
Documents

Highlights
- Automation-specific streamlined design for high-throughput bisulfite conversion of DNA for methylation analysis.
- Compatible with Illumina® Infinium™ MethylationEPIC, HTS iSelect® Methyl Custom, and Mouse Methylation arrays.
- Optimized buffer volumes for consistent automation performance.
Description
Binding Capacity | 1 µg total DNA per 5 µl MagBinding Beads |
---|---|
Conversion Efficiency | > 99.5% of non-methylated C residues are converted to U; > 99.5% protection of methylated cytosines. |
DNA Purity | A260/A280 & A260/A230 ≥ 1.8 |
Input | 100 pg - 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
Input Recovery | > 80% (≥ 100 bp) |
Required Equipment | Thermal cycler with heated lid. |
Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
Supplemental Info | |
User Provided Materials | Ethanol (95 – 100%), non-skirted 96-well microplates, and 96-well microplate sealing films.
See Recommendations for instrument hardware and labware requirements. |
Q1: What is the difference between the EZ DNA Methylation-Lightning Automation Kit and EZ-96 DNA Methylation-Lightning MagPrep Kit?
The EZ DNA Methylation-Lightning automation kit features optimized buffer volumes that allow for consistent high-throughput automation performance.
Q2: Do you offer scripts for instruments not listed in the protocol?
While we do not offer scripts for non-verified instruments, we can provide general technical support for any automation instrument.
Q3: Can I create my own script instead?
Yes, we provide a guideline for users to create their own script in the protocol.
Cat # | Name | Size | Price | |
---|---|---|---|---|
D5032-1 | Lightning Conversion Reagent | 15 ml | €166,10 | |
D5049-3 | M-Binding Buffer | 100 ml | €45,00 | |
D5007-4 | M-Wash Buffer | 36 ml | €45,20 | |
D5046-5 | L-Desulphonation Buffer | 80 ml | €90,40 | |
D5049-6 | M-Elution Buffer | 50 ml | €35,00 | |