EZ DNA Methylation-Lightning Automation Kit

D5049


EZ DNA Methylation-Lightning Automation Kit

Cat # Name Size Price Quantity
D5049 EZ DNA Methylation-Lightning Automation Kit 96 preps €293,10
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EZ DNA Methylation-Lightning Automation Kit

Highlights
  • Automation-specific streamlined design for high-throughput bisulfite conversion of DNA for methylation analysis.
  • Compatible with Illumina® Infinium™ MethylationEPIC, HTS iSelect® Methyl Custom, and Mouse Methylation arrays.
  • Optimized buffer volumes for consistent automation performance.
Description

The EZ DNA Methylation-Lightning Automation Kit is a high-throughput DNA bisulfite conversion kit designed for streamlining automation workflows. It is recommended for several downstream Illumina® Infinium® products such as MethylationEPIC, HTS iSelect® Methyl Custom, and Mouse Methylation BeadChip Arrays. This automation-specific kit features optimized buffer volumes for consistent performance on all open platform liquid handlers and magnetic bead transfer automated systems. Verified automation scripts are readily available for use on the Hamilton Microlab® STAR™/STARlet™, Thermo Fisher Scientific KingFisher™ Flex, and Tecan Fluent®.

For automation scripts and support, email automation@zymoresearch.com.

Binding Capacity 1 µg total DNA per 5 µl MagBinding Beads
Conversion Efficiency > 99.5% of non-methylated C residues are converted to U; > 99.5% protection of methylated cytosines.
DNA Purity A260/A280 & A260/A230 ≥ 1.8
Input 100 pg - 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
Input Recovery > 80% (≥ 100 bp)
Required Equipment Thermal cycler with heated lid.
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info
User Provided Materials Ethanol (95 – 100%), non-skirted 96-well microplates, and 96-well microplate sealing films.

See Recommendations for instrument hardware and labware requirements.

The EZ DNA Methylation-Lightning automation kit features optimized buffer volumes that allow for consistent high-throughput automation performance.

While we do not offer scripts for non-verified instruments, we can provide general technical support for any automation instrument.

Yes, we provide a guideline for users to create their own script in the protocol.

Cat # Name Size Price
D5032-1 Lightning Conversion Reagent 15 ml €166,10
D5049-3 M-Binding Buffer 100 ml €45,00
D5007-4 M-Wash Buffer 36 ml €45,20
D5046-5 L-Desulphonation Buffer 80 ml €90,40
D5049-6 M-Elution Buffer 50 ml €35,00