EZ-96 DNA Methylation MagPrep
D5040 / D5041
- Proven procedure for complete bisulfite conversion of DNA.
- High-throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
The EZ-96 DNA Methylation MagPrep is a bisulfite conversion kit that features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
|Applications||Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.|
|Equipment||Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.|
|Input||500 pg - 2 µg of DNA|
|Processing Time||12-16 hours|
|Sample Source||Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.|
Q1: What leads to poor conversion efficiency/ low yields?
Q2: How to quantify / visualize converted DNA?
Q3: What is the minimum DNA size that can be recovered?
Q4: How long is bisulfite converted DNA stable at -20 °C?
Q5: Does bisulfite conversion only occur in a CpG context?
Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?
Q7: Which polymerase is recommended for amplification from bisulfite-converted DNA?
Q8: Tips for bisulfite primer design?
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