EZ-96 DNA Methylation-Lightning MagPrep

D5046 / D5047

EZ-96 DNA Methylation-Lightning MagPrep

D5046 / D5047

Cat # Name Size Price Quantity
D5046 EZ-96 DNA Methylation-Lightning MagPrep 4 x 96 Rxns. €696,00
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D5047 EZ-96 DNA Methylation-Lightning MagPrep 8 x 96 Rxns. €1.126,00
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Discover the fastest bisulfite conversion kit with the most efficient method for complete bisulfite conversion of DNA for methylation analysis.


  • High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
  • Ready-to-use conversion reagent is added directly to DNA.
  • High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.


The EZ-96 DNA Methylation-Lightning MagPrep Kit is the fastest magnetic bead-based DNA bisulfite conversion kit for high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent - simply add this unique reagent to a DNA sample and incubate the reaction for about one hour. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead-based bisulfite conversion kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR, arrays, library preps, and Next-Generation sequencing.

Technical Specifications

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 100 pg - 2 µg of DNA.
Processing Time 1.5 hours
Recovery > 80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ

Q1: Tips for bisulfite primer design?

Q2: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q3: Does bisulfite conversion only occur in a CpG context?

Q4: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q5: What is the minimum DNA size that can be recovered?

Q6: How to quantify / visualize converted DNA?

Q7: What leads to poor conversion efficiency/ low yields?

Q8: How long is bisulfite converted DNA stable at -20 °C?

Product Video




“It combines the speed of the lightning kit with the use of magnetic beads. This allows the full automation of bisulfite treatment. This is what we were looking for.”

- Marco M. (UCLA)

Kit Components