EZ-96 DNA Methylation-Lightning Kit

D5032 / D5033


EZ-96 DNA Methylation-Lightning Kit

D5032 / D5033

Cat # Name Size Price Quantity
D5032 EZ-96 DNA Methylation-Lightning Kit (Shallow-Well) 2 x 96 Rxns. €442,00
+ -
D5033 EZ-96 DNA Methylation-Lightning Kit (Deep-Well) 2 x 96 Rxns. €442,00
+ -

Documents


Discover the fastest bisulfite conversion kit with the most efficient method for complete bisulfite conversion of DNA for methylation analysis.

Highlights


  • Fastest 96-well bisulfite conversion kit for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
  • Ready-to-use conversion reagent is added directly to DNA.
  • High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.

Description


The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume Deep-well: ≥ 15 µl
Shallow-well: ≥ 30 µl
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 100 pg - 2 µg of DNA.
Processing Time 1.5 hours
Recovery > 80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

Product FAQ


Q1: Tips for bisulfite primer design?

Q2: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q3: Does bisulfite conversion only occur in a CpG context?

Q4: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?

Q5: What is the minimum DNA size that can be recovered?

Q6: How to quantify / visualize converted DNA?

Q7: What leads to poor conversion efficiency/ low yields?

Q8: How long is bisulfite converted DNA stable at -20 °C?

Product Video


Citations


Kit Components