EZ-96 DNA Methylation Kit
D5004 / D5003
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.
|Applications||Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.|
|Elution Volume||≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
|Equipment||Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.|
|Input||500 pg - 2 µg of DNA|
|Processing Time||12-16 hours|
|Sample Source||Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.|
Q1: What leads to poor conversion efficiency/ low yields?
Q2: How to quantify / visualize converted DNA?
Q3: What is the minimum DNA size that can be recovered?
Q4: How long is bisulfite converted DNA stable at -20 °C?
Q5: Does bisulfite conversion only occur in a CpG context?
Q6: Is an incubation with desulphonation buffer for longer than 20 minutes recommended?
Q7: Which polymerase is recommended for amplification from bisulfite converted DNA?
Q8: Tips for bisulfite primer design?
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