EZ-96 DNA Methylation-Direct MagPrep

D5045 / D5044

EZ-96 DNA Methylation-Direct MagPrep

Cat # Name Size Price Quantity
D5045 EZ-96 DNA Methylation-Direct MagPrep 8 x 96 Rxns. €1.375,20
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D5044 EZ-96 DNA Methylation-Direct MagPrep 4 x 96 Rxns. €850,00
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  • High throughput, complete bisulfite conversion of DNA directly from blood, tissue, cells, FFPE and LCM-derived samples
  • Compatible with small sample inputs – as few as 10 cells or 50 pg of DNA.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.

The EZ-96 DNA Methylation-Direct MagPrep is a magnetic bead-based bisulfite conversion kit that allows simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this magnetic bead-based bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. These innovations have been coupled to a magnetic bead-based clean-up for high-throughput methylation analysis. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead based bisulfite conversion kit has been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

For automation scripts and support, email automation@zymoresearch.com

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
Processing Time 4 hours
Recovery > 80%
Sample Source Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.
Supplemental Info

Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and result in lower yields.

Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.

ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).

> 50 bp.

Following bisulfite treatment of genomic DNA, nonmethylated cytosine residues are converted into uracil. The recovered DNA is typically A, U, and T-rich. The original base-pairing no longer exists. Instead, it is single-stranded with limited non-specific base-pairing at room temperature. The absorption coefficient at 260 nm resembles that of RNA. Use a value of 40 μg/ml for Ab260 = 1.0 when determining the concentration of the recovered bisulfite-treated DNA. To visualize, run converted DNA on agarose gel then chill the gel on ice for 30 minutes. The expected smear will be between 100-1500bp.

Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.

Converted DNA should be eluted in M-Elution Buffer to keep the converted DNA stable for long term storage. If stored properly for long term (<-20C), the samples should last longer than a month. Minimize freeze/thawing to keep the bisulfite converted DNA stable.

Cat # Name Size Price
D4100-5-8 MagBinding Beads 8 mL €92,00
D4100-5-16 MagBinding Beads 16 mL €166,00
C2003 Elution Plate 2 Plates €21,30
C2002 Collection Plate 2 Plates €24,70
C2005 Conversion Plate w/ Cover Foil 2 Plates/Foils €10,20
D5021-7 M-Solubilization Buffer 18 ml €34,20
D5021-8 M-Reaction Buffer 4ml €25,70
D5021-9 M-Digestion Buffer (2X) 15 ml €18,40
D5040-3 M-Binding Buffer 250 ml €128,30
D5040-5 M-Desulphonation Buffer 80 ml €90,40
D5040-4 M-Wash Buffer 72 ml €83,10
D5007-6 M-Elution Buffer 8 ml €12,30
D5006-2 M-Dilution Buffer-Gold 7 ml €12,30
D5003-1 CT Conversion Reagent (96 Conversions) 1 Bottle €77,00