Direct-zol RNA Miniprep Plus Kits
R2072 / R2071 / R2070 / R2073 / R2070T
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2071, R2073) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||100 µg RNA (binding capacity), ≥ 50 µl (elution volume)|
Q1: Is Direct-zol suitable for very small numbers of cells?
Q2: Is DNase I available for individual purchase?
Q3: How to store DNase-I following resuspension?
Q4: Is the DNase-I treatment necessary?
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q10: Which phenol-based reagents are compatible with Direct-zol?
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Q13: I ran out of RNA Wash Buffer. Can I use something else?
“It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
- A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
- Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
- Arjan V. (Indiana University)