Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057


Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057

Cat # Name Size Price Quantity
R2054 Direct-zol-96 RNA 2 x 96 preps €418,00
+ -
R2055 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 1 TRI Reagent) 2 x 96 preps €628,00
+ -
R2056 Direct-zol-96 RNA 4 x 96 preps €675,00
+ -
R2057 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps €1.105,00
+ -

Documents


Isolate total RNA and small/miRNA from TRIzol without phase separation

Highlights


  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.

Description


The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).

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Technical Specifications


Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microplate centrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥10 µl (elution volume)

Product FAQ


Q1: Is DNase I available for individual purchase?

Q2: How to store DNase-I following resuspension?

Q3: Is the DNase-I treatment necessary?

Q4: Is the kit compatible with samples stored in DNA/RNA Shield?

Q5: Is Direct-zol suitable for very small numbers of cells?

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Q10: Which phenol-based reagents are compatible with Direct-zol?

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Product Video


Citations


Reviews


“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

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Kit Components