Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057

Direct-zol-96 RNA Kits

Cat # Name Size Price Quantity
R2054 Direct-zol-96 RNA 2 x 96 preps €418,00
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R2055 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 1 TRI Reagent) 2 x 96 preps €651,00
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R2056 Direct-zol-96 RNA 4 x 96 preps €675,00
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R2057 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps €1.151,00
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Direct-zol-96 RNA Kits


  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.

The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).

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Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microplate centrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥10 µl (elution volume)

Q1: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q2: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q3: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q4: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q5: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol.

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q10: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Q14: What is the average RNA yield? (chart)

Input Average RNA Yield
Cells 10 µg (per 106 cells)
HeLa 15 µg
High Yield Tissue (mouse) ≥ 30 µg (per 10 mg)
Spleen 30-50 µg
Liver 40-60 µg
Low Yield Tissue (mouse) ≤ 30 µg (per 10 mg)
Brain, Heart 5-15 µg
Muscle 5-20 µg
Lung 10-20 µg
Intestine 10-30 µg
Kidney 20-30 µg
Whole Blood (per 1 ml)
Porcine 10-20 µg
Human 2-10 µg

Q15: How to improve purity, RIN scores and eliminate contamination (i.e., A260/230, A260/280 ratios, DNA, phenol, protein, salt, etc.)?

Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

Q16: How to improve RNA yield?

  • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
  • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).

“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

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Cat # Name Size Price
E1010-1-16 DNA Digestion Buffer 16 mL €29,00
R2050-1-200 TRI Reagent 200 ml €238,00
E1010-1-4 DNA Digestion Buffer 4 mL €15,00
C2003 Elution Plate 2 Plates €21,00
C2002 Collection Plate 2 Plates €23,00
C2007-2 96-Well Plate Cover Foil 2 Foils €12,00
C2007-4 96-Well Plate Cover Foil 4 Foils €12,00
C2004 Zymo-Spin I-96 Plate 2 Plates €149,00
W1001-30 DNase/RNase-Free Water 30 ml €22,00
W1001-10 DNase/RNase-Free Water 10 ml €19,00
R2050-2-160 Direct-zol RNA PreWash (Concentrate) 160 ml €166,00
R2054 Direct-zol-96 RNA 2 x 96 preps €418,00
R2056 Direct-zol-96 RNA 4 x 96 preps €675,00
R1003-3-48 RNA Wash Buffer 48 ml €105,00
E1010 DNase I Set 250 U €64,00