Zymoclean Large Fragment DNA Recovery

D4045 / D4046


Zymoclean Large Fragment DNA Recovery

D4045 / D4046

Cat # Name Size Price Quantity
D4045 Zymoclean Large Fragment DNA Recovery 25 Preps €92,00
+ -
D4046 Zymoclean Large Fragment DNA Recovery 100 Preps €298,00
+ -

Documents


Highly efficient large DNA purification from agarose gels.

Highlights


  • Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
  • Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
  • Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.

Description


The Zymoclean Large Fragment DNA Recovery Kit is a gel DNA extraction kit that provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.

Technical Specifications


Applicable For High-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications
Elution Volume ≥ 10 µl
Equipment Microcentrifuge
Purity High-quality, purified DNA is especially well-suited for sequencing and ligation reactions
Sample Source Agarose gels containing large DNA fragments of interest
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Size Range 50 bp to 200 kb
Type Large DNA
Yield Up to 10 µg DNA

Citations


Reviews


“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”

- Ingrid M.

“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”

- Kelly-Anne F.

“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“

- Thomas B (University of Tennessee, Knoxville)

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Kit Components