ZR-96 DNA Sequencing Clean-up Kit

D4052 / D4053


ZR-96 DNA Sequencing Clean-up Kit

Cat # Name Size Price Quantity
D4052 ZR-96 DNA Sequencing Clean-up Kit 2 x 96 Preps €249,70
- +
D4053 ZR-96 DNA Sequencing Clean-up Kit 4 x 96 Preps €398,60
- +

Documents



Highlights

  • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
  • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
  • Reusable!
Description

The ZR-96 DNA Sequencing Clean-Up Kits provides a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.


Elution Volume ≥ 15 µl of DNA Elution Buffer
Equipment Centrifuge with microplate carriers
Sample Source Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Yield Up to 5 µg DNA



Cat # Name Size Price
D4050-1-55 Sequencing Binding Buffer 55 ml €106,40
D4050-2-70 Sequencing Wash Buffer 70 ml €58,30
C2008 96-Well PCR Conversion Plate 2 Plates €10,20
C2002 Collection Plate 2 Plates €24,70
C2006 Zymo-Spin IB-96 Plate 2 Plates €156,80