ZR-96 DNA Sequencing Clean-up Kit

D4052 / D4053


ZR-96 DNA Sequencing Clean-up Kit

D4052 / D4053

Cat # Name Size Price Quantity
D4052 ZR-96 DNA Sequencing Clean-up Kit 2 x 96 Preps €206,00
+ -
D4053 ZR-96 DNA Sequencing Clean-up Kit 4 x 96 Preps €347,00
+ -

Documents


Reusable and simple purification kit for preparing sequence-ready DNA.

Highlights


  • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
  • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
  • Reusable!

Description


The ZR-96 DNA Sequencing Clean-Up Kits provides a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.

Technical Specifications


Elution Volume ≥ 15 µl of DNA Elution Buffer
Equipment Centrifuge with microplate carriers
Sample Source Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products
Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
Yield Up to 5 µg DNA

Citations


Kit Components