ZR-96 DNA Clean-Up Kit

D4017 / D4018


ZR-96 DNA Clean-Up Kit

D4017 / D4018

Cat # Name Size Price Quantity
D4017 ZR-96 DNA Clean-Up Kit (Shallow Well) 2 x 96 Preps €220,00
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D4018 ZR-96 DNA Clean-Up Kit (Shallow Well) 4 x 96 Preps €420,00
+ -

Documents


Clean-up PCR and other enzymatic reactions in as little as 2 minutes

Highlights


  • Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
  • ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.

Description


The ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.

Technical Specifications


Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume ≥ 30 µl for shallow well, ≥ 10 µl for deep well
Equipment Centrifuge with microplate carriers
Purity A260/A280 > 1.8
Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range 75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well
Yield ≤ 5 µg total DNA can be recovered. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Product FAQ


Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Q2: How to process naked DNA stored in DNA/RNA Shield?

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Q5: How many times can columns be reloaded?

Q6: What is the minimum input volume of DNA sample?

Kit Components