ssDNA/RNA Clean & Concentrator

D7010 / D7011


ssDNA/RNA Clean & Concentrator

D7010 / D7011

Cat # Name Size Price Quantity
D7010 ssDNA/RNA Clean & Concentrator 20 Preps €89,00
+ -
D7011 ssDNA/RNA Clean & Concentrator 50 Preps €179,00
+ -

Documents


Separation of single from double-stranded nucleic acids

Highlights


  • Quick and Reliable: 10 minute clean-up and concentration of ssDNA/RNA (17-200 nt).
  • Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
  • Clean and Pure: ssDNA/RNA ready for downstream applications like PCR, RT-qPCR, etc.

Description


The ssDNA/RNA Clean & Concentrator provides a simple and reliable method for the rapid separation, clean-up, and concentration of 10 µg per prep of single stranded DNA and/or RNA (e.g., short transcripts, probes, primers) from double stranded species (e.g., genomic DNA).This simple 10 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology. Single stranded DNA or RNA ≥ 17 to 200 nucleotides (e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highly concentrated, purified ssDNA/RNA that is suitable for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.

Technical Specifications


Equipment Microcentrifuge
Purity DNA/RNA is ready for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.
A260/280 > 1.8
A260/230 > 1.8
Sample Source Mixtures of double and single stranded DNA and RNA species with single stranded fragments 17 to 200 nucleotides (e.g., short transcripts, probes, primers)
Size Range DNA and/or RNA 17-200 nt
Yield 10 µg DNA/RNA

Citations


The ssDNA/RNA Clean & Concentrator was used to separate single-stranded RNA from total RNA for gene expression analysis in Diaphorina citri.

Hajeri, S et al. Citrus tristeza virus-based RNAi in citrus plants induces gene silencing in Diaphorina citri, a phloem-sap sucking insect vector of citrus greening disease (Huanglongbing). Journal of Biotechnology. 2014.

Biotinylated CD4 ssDNA aptamers were mixed with streptavidin and this conjugate was purified with the ssDNA/RNA Clean & Concentrator to remove free streptavidin. Functionality of aptamer polymers showed significant blocking (up to 70% inhibition) of binding of viral gp120 to CD4 expressing cells.

Zhao, N et al. Blocking interaction of viral gp120 and CD4-expressing T cells by single-stranded DNA aptamers. The International Journal of Biochemistry & Cell Biology. 2014.

In vitro transcribed biotinylated transcripts were purified using the ssDNA/RNA Clean & Concentrator for biotin pull-down assay and subsequent western blotting of the RNA-protein complexes.

Kuwano, Y et al. Transformer 2β and miR-204 regulate apoptosis through competitive binding to 3’ UTR of BCL2 mRNA. Cell Death & Differentiation. 2015.

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Kit Components