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    Quick-RNA Midiprep Kit

    R1056


    Quick-RNA Midiprep Kit

    Cat # Name Size Price Quantity
    R1056 Quick-RNA Midiprep Kit 25 preps €319,40
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    Highlights
    • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
    • High Yield: Recover up to 1 mg of RNA
    • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
    Description

    The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.

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    Equipment Microcentrifuge, vortex, vacuum manifold
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Cells or tissue samples, yeast, plant or bacteria.
    Size Range Total RNA ≥ 17 nt
    Supplemental Info
    Yield Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)

    Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).

    Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

    Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.

    "RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

    - Lisa G. (University of Virginia)

    “Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

    - Erin B. (Occidental College)

    "Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

    - Xiaolu J. (Florida Atlantic University)

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    Cat # Name Size Price
    W1001-10 DNase/RNase-Free Water 10 ml €21,90
    C1001-50 Collection Tubes 50 Pack €17,40
    R1003-3-6 RNA Wash Buffer 6 ml €20,80
    R1020-1-100 ZR RNA Buffer 100 ml €161,50
    R1020-2-12 RNA Pre-Wash Buffer 12 ml €24,30

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