Quick-RNA Fungal/Bacterial Microprep Kit
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
|Applicable For||For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.|
|Elution Volume||≥ 6 µl|
|Equipment||Microcentrifuge, vortex, cell disrupter/pulverizer|
|Processing Time||≤ 10 minutes|
|Processing Volume||10-20 mg (wet weight) fungi or bacteria, 2x108 bacterial cells, or 2x107 yeast cells|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8.
|Sample Source||Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.|
|Size Range||Total RNA ≥ 17 nt|
10 µg RNA (binding capacity)
≥ 6 µl (elution volume)