Quick-DNA Tissue/Insect 96 Kit
- Simple, high-throughput (96-well) method for efficient isolation of DNA from insects, including mosquitoes, bees, lice, ticks, and D. melanogaster .
- Compatible with tough-to-lyse tissues from other organisms.
The Quick-DNA Tissue/Insect 96 Kit is an insect DNA extraction kit designed for the simple, high-throughput (96-well) method for the rapid isolation of DNA (e.g., genomic, viral, mitochondrial) from fresh, frozen, or stored insect specimens including mosquitoes, bees, lice, ticks, and D. melanogaster . The procedure is easy and can be completed in minutes: samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. The DNA is then isolated and purified using our Zymo-Spin technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood, and cultured cells.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation sequencing.|
|Elution Volume||≥ 25 µl|
|Equipment||Centrifuge with microplate carriers, 96-well plate/block disruptor or pulverizer.|
|Purity||A260/A280 nm ≥1.8.|
|Sample Source||Insect or solid tissue|
|Sample Storage||DNA stored at ≤ -20°C.|
|Size Range||Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.|
|Yield||5 µg total DNA|
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
Q2: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Q3: When can an RNase A treatment be implemented in the protocol?
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