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    Quick-DNA/RNA HT

    R2150 / R2151 / R2150-E / R2151-E


    Quick-DNA/RNA HT

    Cat # Name Size Price Quantity
    R2150 Quick-DNA/RNA HT 250 Preps €328,10
    - +
    R2151 Quick-DNA/RNA HT 1000 Preps €1.196,80
    - +
    R2150-E Quick-DNA/RNA HT – Dx 250 Preps €328,10
    - +
    R2151-E Quick-DNA/RNA HT – Dx 1000 Preps €1.196,80
    - +

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    Highlights
    • High-throughput, magnetic-bead based purification of total nucleic acid (DNA/RNA) from host, plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal and biopsy samples.
    • High-quality DNA/RNA is ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
    Description

    The Quick-DNA/RNA HT kit is intended for rapid, high-throughput nucleic acid extraction (automated or manual) from any biological or clinical sample (e.g., swabs – nasal/nasopharyngeal, oropharyngeal, etc.; biological liquids – blood, plasma, serum, saliva, sputum, cells in suspension, etc.; tissue – needle biopsies, LCM, etc.) and/or samples stored in most collection matrices and devices (e.g., UTM, VTM, *DNA/RNA Shield™, RNAlater, RNAProtect, etc.). 

    The kit is compatible with robotic-type sample processors (i.e, bead movers) in combination with sensitive downstream molecular amplification assays.  High-quality DNA/RNA extracted with the Quick-DNA/RNA HT kit can be used for Next-Gen sequencing, RT/qPCR and more.

    Binding Capacity 5 µg DNA/RNA per 10 µl MagBinding Beads
    Elution Volume ≥ 15 µl DNase/RNase-Free Water
    Purity DNA/RNA is ready for Next-Gen Sequencing, RT/qPCR, etc.
    Sample Sources Any biological sample (e.g., swabs, liquids, cells, tissue, etc.), and samples collected/stored in media (e.g., UTM, VTM, saline, PBS, DNA/RNA Shield™, PAXgene, RNAlater, RNAProtect, etc.)
    Storage Temperature and Stability
    • Store all components (i.e., buffers/reagents, columns) at room temperature (20-30°C).
    • Eluted DNA/RNA can be used immediately or stored frozen (-20/-80°C).

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Yes, the Quick-DNA/RNA HT – Dx (R2150-E, R2151-E) is CE-IVD marked.

    Virus detection is one of the dominant applications for this kit. Other dominant applications include infectious disease, microbiomics, cancer research, and more.

    Yes, there are no restrictions to this kit on veterinary samples.

    No. This kit extracts total nucleic acids (DNA & RNA) together in the same fraction.

    Total nucleic acid (DNA/RNA) extracted can be directly used for any DNA application. For RNA applications, include DNase I treatment if it is necessary to the downstream analysis.

    The Quick-DNA/RNA HT kit has a broader spectrum of sample inputs and applications. It is recommended for clinical type samples. In comparison to the Quick-DNA/RNA™ Viral MagBead (R2140, R2141, R2140-E, R2141-E), the HT family provides a more general, streamlined workflow targeted toward (mainly) new customers in the clinical diagnostics field, while the prior focuses on (mainly) viral samples in research settings.

    Yes, it is recommended to remove RNAlater prior to processing. However, when removal is not possible, the input should be diluted. For example, use 50 µl of input per prep and add 750 µl of DNA/RNA Buffer HT at step 1 under (III) DNA/RNA Purification on page 7 in the instruction manual.

    If the input is in TRIzol or TRI-Reagent, it is recommended to add chloroform and process the sample to produce the aqueous phase. Carefully, transfer the aqueous phase into a new tube and process with the kit (see sample preparation). To process input in TRIzol or TRI-Reagent directly, it is recommended to use the Direct-zol-96 MagBead RNA kit (R2100, R2101, R2102, R2103).

    For unbiased bacterial detection, proper homogenization with the ZR BashingBead Lysis Tubes (S6012-50) is recommended before continuing to nucleic acid extraction with the kit.

    This kit can be directly used with all robotic-type sample processors (i.e., bead movers) for nucleic acid extraction from clinical samples. Detailed specifications and steps can vary based on sample type and automation platform used. For automation scripts and related technical support, please email automation@zymoresearch.com for free consultation.

    Compatible automated machines include, but are not limited to:

    • Allsheng Autopure 
    • Accuris Isopure 96
    • Kingfisher Flex
    • Kingfisher Apex
    • Tecan Fluent
    • Hamilton Microlab Star
    • Opentrons OT-2

    Additionally, the kit is also compatible with 8-16 prep platforms, such as:

    • KingFisher Duo/Duo Prime
    • IsoPure Mini

    Please note that only the standard format (R2150, R2150-E, R2151, R2151-E) of this kit is compatible with these smaller platforms, as the Load N’ Go format (R2152) is only compatible with 96-prep format platforms.

    We can conduct proof-of-principle testing to validate the kit’s chemistry on any platform we have in-house. If we do not have the preferred platform at our disposal, we can send a free kit for the purpose of workflow validation and provide support with scripting and troubleshooting as needed at no cost.

    Cat # Name Size Price
    R2150-1-100 DNA/RNA Buffer HT 100 mL €182,50
    D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg €50,80
    D3001-2-60 Proteinase K w/ Storage Buffer Set 60 mg €152,30
    D4100-2-6 MagBinding Beads 6 ml €93,20
    D4100-2-8 MagBinding Beads 8 ml €124,10
    R2130-1-30 MagBead DNA/RNA Wash 1 30 ml €72,70
    R2130-1-120 MagBead DNA/RNA Wash 1 120 ml €228,30
    R2130-2-20 MagBead DNA/RNA Wash 2 20 ml €62,30
    R2130-2-80 MagBead DNA/RNA Wash 2 80 ml €197,20
    W1001-30 DNase/RNase-Free Water 30 ml €25,40

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