Quick-DNA FFPE Miniprep


Quick-DNA FFPE Miniprep

Cat # Name Size Price Quantity
D3067 Quick-DNA FFPE Miniprep 50 Preps €180,00
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Quick-DNA FFPE Miniprep

Highlights

  • Streamlined purification of high-quality FFPE tissue DNA that is ideal for PCR, Next-Gen library prep, enzymatic manipulations, etc.
  • Size selection technology; recover total DNA >50 bp or >500 bp.
Description

The Quick-DNA FFPE Kit provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Zymo-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as described in the protocol. PCR inhibitors are effectively removed during the isolation procedure and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.


Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment Microcentrifuge, heat block/bath (55°C and 90°C), isopropanol, beta-mercaptoethanol (optional).
Processing Volume Tissue - ≤25 mg or (4) 20 µm thick with a surface area of 20 mm2
Purity Typical A260/A280 ≥ 1.8
Sample Source Tissue from paraffin block or tissue sections. Compatible with fresh/frozen tissue specimens
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Size Range > 50 bp
Type Total DNA
Yield Up to 25 µg total DNA can be eluted into ≥ 50 µl.

Cat # Name Size Price
D3050-1-5 2X Digestion Buffer - 5 mL 5 mL €10,00
C1011-50 Zymo-Spin IIC Columns 50 Pack €58,00
C1001-50 Collection Tubes 50 Pack €16,00
D3067-1-20 Deparaffinization Solution 20 ml €22,00
D3067-2-25 Genomic DNA Wash 1 25 ml €22,00
D3067-3-12 Genomic DNA Wash 2 12 ml €18,00
D3004-1-50 Genomic Lysis Buffer 50 ml €36,00
D3004-4-10 DNA Elution Buffer 10 ml €15,00