Quick-cfDNA Serum & Plasma Kit
- High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
- Highest Yields: Consistently purify > 30% more cfDNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.
|DNA Purity||High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.|
|DNA Recovery||Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.|
|DNA Storage||Eluted DNA should be stored at ≤ -20ºC|
|Processing volume||Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m
l Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml
|Recovery Volume||≥ 35 µl of DNA Elution Buffer or DNase free water.|
|Required Equipment||Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge.|
|Sample Sources||Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).|
Q1: Is the kit compatible to Streck tubes and PAXgene cfDNA tubes?
Q2: How to process samples stabilized in DNA/RNA Shield with the kit?
Preanalytical blood sample workup for cell‐free DNA analysis using Droplet Digital PCR for future molecular cancer diagnosticsvan Ginkel, J. H. (2017). Preanalytical blood sample workup for cell-free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics. Cancer Medicine. 6(10), 2297-2307.
Blood Collection and Cell-Free DNA Isolation Methods Influence the Sensitivity of Liquid Biopsy Analysis for Colorectal Cancer DetectionBarták, B. K. (2018). Blood Collection and Cell-Free DNA Isolation Methods Influence the Sensitivity of Liquid Biopsy Analysis for Colorectal Cancer Detection. Pathology & Oncology Research.
T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body FluidsNai, Y. (2017). T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids. Scientific Reports, 7(1).
Flexibil[e] for input volumes [with] quick references.”
- E. L. (Cepheid)
“Easy to use and good yield.”
- X.Q (University of California, San Diego)
“Higher DNA concentration in the final eluate from the Zymo kit when compared to the Qiagen kit. Total yield, measured by reference genes, is higher for the Zymo kit when compared to the Qiagen kit.”
- T.R. (Aarhus University Hospital)