Proteinase K w/ Storage Buffer Set


Proteinase K w/ Storage Buffer Set

Cat # Name Size Price Quantity
D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg Contact For Pricing
D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg Contact For Pricing

Documents


Proteinase K is used in molecular biology applications to digest unwanted proteins such as nuclease

Highlights

  • Lyophilized enzyme provided with Proteinase K Storage Buffer.
Description

Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.


Enzyme Commission Number (EC 3.4.21.64)
pH and Temperature Range 4.0 to 12.0 (8.0 is optimum), 25 to 65°C
Source Engyodontium album
Specific Activity > 30 units/mg protein
Unit Definition One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.