Proteinase K w/ Storage Buffer Set
D3001-2-5 / D3001-2-20 / D3001-2-60 / D3001-2-125
- Lyophilized enzyme provided with Proteinase K Storage Buffer.
Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.
|Enzyme Commission Number||(EC 18.104.22.168)|
|pH and Temperature Range||4.0 to 12.0 (8.0 is optimum), 25 to 65°C|
|Specific Activity||> 30 units/mg protein|
|Unit Definition||One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.|