Mix & Go! E.coli Transformation Kit and Buffer Set
T3001 / T3002
- Easy 3 Step Protocol: Produce reliable chemically competent E. coli in less than 45 minutes.
- Simple 20 sec Transformation: No heat shock! Just add DNA and spread.
- High Transformation Efficiencies: Achieve 108-109 transformants per µg of plasmid DNA.
|Compatible Strains||The Kit is optimized to work with K12 and B derivative strains. Some commonly used strains include, TOP10, ccdB survival, XL1 Blue and Stbl3.|
|Processing Time||≤ 45 minutes|
|Product Storage||Please store the Wash Buffer (2X), Competent Buffer (2X), and Dilution Buffer at 0-8°C. The ZymoBroth can be stored at room temperature.|
|Transformation Efficiency||108-109 transformants per µg of plasmid DNA for most common lab strains.|
Q1: Which E. coli strains can be used with the Transformation Kit?
Q2: Are there any specific considerations in preparing of overnight culture?
Q3: How long can the cells sit in 1X Wash buffer?
Q4: How long can the cells sit in 1X Competent buffer?
Q5: How can I boost transformation efficiencies?
Q6: I accidentally stored the kit at room temperature. Is it still okay to use?
Q7: What are the best practices for aliquoting the competent cells?
Q8: Can competent cells be stored in liquid nitrogen (N2)?
Q9: Is the transformation efficiency of 108 -109 guaranteed for the Mix & Go! E..coli Transformation Kit & Buffer Set.
Z-competent DH5α E. coli cells were prepared using the Mix & Go E. coli Transformation Buffer Set from Zymo Research and used for high-efficiency transformations in a study that tested the strength and compatibility of varying transcription terminators, which often need to be used in series to stop transcription originating from the strong promoters required in synthetic circuits. Data from this study highlighted 39 terminators that not only have the ability to reduce downstream expression by >50-fold, but also have enough sequence diversity to be used together.Chen, Y. et al. (2013) Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature Methods 10 (2013): 659-664.
To determine the structural requirements for a catalytically active histidine triad nucleotide protein (ecHinT) that allows Escherichia coli to grow on D-alanine as a sole carbon source, the authors designed various plasmids that encoded mutant Hint proteins. Competent HinT-deficient E. coli were prepared using the Mix & Go E. coli Transformation Kit from Zymo Research and were then transformed using the designed plasmids. The authors found that the C-terminal loop of ecHinT is an important structure of an active ecHinT.Bardaweel, S. et al. (2011) E. coli histidine triad nucleotide binding protein 1 (ecHinT) is a catalytic regulator of D-alanine dehydrogenase (DadA)