The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a “spike-in” control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration. The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam–, Dcm–), and the methylated pUC19 DNA is pUC19 enzymatically methylated at all cytosines in the dinucleotide sequence 5′...CpG...3′ by CpG Methylase.

Additional Info	pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.
Concentration	1 ng/µl
Storage	≤ -20°C